- Fluorenone based fluorescent probe for selective "turn-on" detection of pyrophosphate and alanine.
Fluorenone based fluorescent probe for selective "turn-on" detection of pyrophosphate and alanine.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2017-11-21)
Daniel Thangadurai T, Nithya I, Manjubaashini N, Bhuvanesh N, Bharathi G, Nandhakumar R, Nataraj D
PMID29153841
ABSTRACT
To sense biologically important entities with different size and dimensions, a fluorenone based fluorescent receptor was designed and synthesized. Probe 1 displayed a distinct fluorescence enhancement emission at 565nm for pyrophosphate and 530nm for alanine in polar solvent. The fluorescence titration experiments confirm 1:1 stoichiometric ratio with high-binding constant and very low limit of detection (LoD) values. Receptor 1 showed a highly selective and sensitive recognition to HP2O73- and to alanine over other competitive anions and amino acids. In addition, the fluorescence lifetime measurement and reversible binding study results support the practical importance of 1.
MATERIALS