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Nucleic Acid Electrophoresis Protocols & Introduction
An introduction to Nucleric Acid electrophoresis and standard protocols for Agarose gel electrophoresis for DNA, RNA & polyacrylamide gel electrophoresis for DNA.
Applying Equilibrated Immobiline® DryStrip Gels
This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.
Preparing for IEF
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System
Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.
Cleaning Up Samples using 2-D Clean-Up Kit
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System
Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.
Inserting Gels into Ettan™ DALT Electrophoresis Units
This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.
Paper-Bridge Loading
This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products
Rehydration Solutions
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
Formamide Molecular Biology Reagent
Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.
Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
DNA Detection
Nancy-520 for DNA Detection and Quantitation
Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels
This page shows how to apply equilibrated Immobiline DryStrip gels
Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
Components of Rehydration Solution
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions
Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.
TAE and TBE Running Buffers Recipe & Video
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Precast Agarose Gels for RNA Electrophoresis
Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.
Brilliant Blue G – Colloidal Concentrate Protocol
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
ReadyBlue Protein Gel Staining Protocols
ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.
Northern & Southern Blot Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Two-Dimensional Separation of Protein Samples
This page describes two-dimensional separation of protein samples with Cytiva products.
Gel Electrophoresis
Protein gel electrophoresis methods are used for protein separation in preparation for downstream applications like Western blotting and mass spectrometry.
Protein Staining Reagents Selection Guide
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
mPAGE®Lux Casting System for SDS-PAGE Gels
Refresh your gel casting workflow with ready-to-use SDS-PAGE gels in just 3 minutes. Discover faster, simpler, and safer gel casting methods.
Bis Tris Polyacrylamide Gel Electrophoresis Technology
Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.
Water for Protein Electrophoresis & Western Blotting
Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
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