A274

Monoclonal Anti-H⁺/K⁺ ATPase (β Subunit) antibody produced in mouse

clone 2G11, ascites fluid, buffered aqueous solution

• MDL number: MFCD02096517
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 2G11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 60-80 kDa (glycosylated form)
• species reactivity: pig, mouse, rabbit, rat, bovine, ferret, canine
• technique(s): immunohistochemistry (frozen sections): suitable; indirect immunofluorescence: 1:2,000; western blot (chemiluminescent): 1:4,000
• isotype: IgG1
• UniProt accession no.: P18597
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Atp4b(11945) ; rat ... Atp4b(24217)

Description

General description

The H⁺/K⁺-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H⁺/K⁺-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H⁺/K⁺ ATPase (β subunit) binds to H⁺/K⁺-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.

The antibody reacts with various forms of the H⁺/K⁺ ATPase β subunit. It binds an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the β subunit. The antibody has been shown to inhibit the enzymatic activity of the H⁺/K⁺-ATPase and to alter the affinity of the cytoplasmic K⁺ binding site. It may be used to localize and detect H⁺/K⁺ ATPase β subunit.

Immunogen

core 34 kDa H⁺/K⁺ ATPase β subunit from deglycosylated pig gastric microsomes.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-H⁺/K⁺ ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves