HTS241RTA
Ready-to-Assay SUCNR1 / GPR91 Succinate Receptor Frozen Cells
About This Item
biological source
human
manufacturer/tradename
Ready-to-Assay
technique(s)
calcium flux assay: suitable
NCBI accession no.
detection method
fluorometric
General description
SUCNR1, or GPR91, is a class A G-protein coupled receptor that binds selectively to succinate, an intermediate in the citric acid cycle, and couples to both the Gi/Go and Gq pathways. Renal proximal tubules are a major site of GPR91 expression, and succinate-mediated activation of renal GPR91 stimulates the release of renin to cause vasoconstriction and hypertension (He et al., 2004). In addition, GPR91 in retinal neurons mediates retinal vascularization induced by succinate, which accumulates in response to hypoxia (Sapieha et al., 2008). Dendritic cells also highly express GPR91, which mediates succinate-induced immune cell migration and activation in vitro and in vivo (Rubic et al., 2008). Therefore, suppression of GPR91 is a potential strategy for treatment of vascular retinopathies, hypertension, and inflammation. Millipore′s cloned human GPR91 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR91 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at GPR91.
Cell Line Description
- GPCR Cell Lines
- Host cells: Chem-1
Application
Biochem/physiol Actions
- GPCR Class: A
- Protein Target: SUCNR1/GPR91
- Target Sub-Family: Succinate
Components
Fifty (50) mL of Media Component.
Disclaimer
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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