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HTS241RTA

Sigma-Aldrich

Ready-to-Assay SUCNR1 / GPR91 Succinate Receptor Frozen Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
Pricing and availability is not currently available.

biological source

human

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

detection method

fluorometric

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

SUCNR1, or GPR91, is a class A G-protein coupled receptor that binds selectively to succinate, an intermediate in the citric acid cycle, and couples to both the Gi/Go and Gq pathways. Renal proximal tubules are a major site of GPR91 expression, and succinate-mediated activation of renal GPR91 stimulates the release of renin to cause vasoconstriction and hypertension (He et al., 2004). In addition, GPR91 in retinal neurons mediates retinal vascularization induced by succinate, which accumulates in response to hypoxia (Sapieha et al., 2008). Dendritic cells also highly express GPR91, which mediates succinate-induced immune cell migration and activation in vitro and in vivo (Rubic et al., 2008). Therefore, suppression of GPR91 is a potential strategy for treatment of vascular retinopathies, hypertension, and inflammation. Millipore′s cloned human GPR91 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR91 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at GPR91.

Cell Line Description

  • GPCR Cell Lines
  • Host cells: Chem-1

Application

Human SUCNR1 / GPR91 GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

  • GPCR Class: A
  • Protein Target: SUCNR1/GPR91
  • Target Sub-Family: Succinate

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.

Disclaimer


This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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