A Prototype Evanescent Wave-Coupled Cavity Ring-down Spectrometer for Probing Real-Time Aggregation Kinetics of Gold and Silver Nanoparticles.

We report on the development of a simple, linear optical cavity-based system combining evanescent wave (EW) with high-sensitive cavity ring-down spectroscopy (CRDS) technique using a diode laser at 644 nm and a right-angled prism for evanescent field generation on prism surface. We characterized the setup in detail and achieved an optimum ring-down time of 159.4 ns and a minimum absorption coefficient of αmin = 1.67 × 10-6 cm-1. We utilized this setup to investigate the salt-induced aggregation kinetics of gold (Au) and silver (Ag) nanoparticles (NPs) at the prism interface with high-sensitivity. We evaluated the extinction rates on the surface due to Au and Ag NPs aggregation and examined the variations due to their respective concentrations. To demonstrate the applicability of the developed EW-CRDS prototype setup to different molecular systems, we investigated the urease-bound aggregation kinetics of the Au and Ag NPs which has not been explored earlier by this linear cavity geometry. We finally illustrated the aggregation dynamics through surface imaging, thus demonstrating an alternative analytical approach to monitor interfacial phenomena using EW-CRDS technique.