229253

Carminic acid

≥90% (HPLC)

• Synonym(s): Natural Red 4
• Empirical Formula (Hill Notation): C₂₂H₂₀O₁₃
• CAS Number: 1260-17-9
• Molecular Weight: 492.39
• Colour Index Number: 75470
• Beilstein: 71651
• EC Number: 215-023-3
• MDL number: MFCD00167028
• UNSPSC Code: 12171500
• PubChem Substance ID: 24853740
• NACRES: NA.47

Properties

• Quality Level: 100
• Assay: ≥90% (HPLC)
• form: powder
• optical activity: [α]20/D +3.1°, c = 1 in H₂O
• color: purple to red-purple; red to very dark red
• mp: 136 °C
• solubility: 1 N acetic acid: 1%, clear to hazy, red
• λmax: 495 nm
• ε (extinction coefficient): ≥13000 at 222-228 nm in ethanol at 0.03 g/L; ≥18000 at 275-281 nm in ethanol at 0.03 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1c(C(O)=O)c(O)cc2C(=O)c3c(O)c(O)c([C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)c(O)c3C(=O)c12
• InChI: 1S/C22H20O13/c1-4-8-5(2-6(24)9(4)22(33)34)13(25)10-11(15(8)27)16(28)12(18(30)17(10)29)21-20(32)19(31)14(26)7(3-23)35-21/h2,7,14,19-21,23-24,26,28-32H,3H2,1H3,(H,33,34)/t7-,14-,19+,20-,21+/m1/s1
• InChI key: DGQLVPJVXFOQEV-JNVSTXMASA-N

Description

General description

Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilized in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.

Application

Carminic acid (CI 75470) is used in Orth′s lithium carmine technique for nuclear staining, Best′s stain for glycogen, and Southgates′s mucicarmine method.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves