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ESG2206

Sigma-Aldrich

ESGRO® Recombinant Rat LIF Protein

ESGRO Leukemia Inhibitory Factor (LIF) supplement for rat ES cell culture. Each vial contains 10^6 units/ml

Synonym(s):

Rat LIF

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

100

Assay

>85% (active component rat LIF (rtLIF), SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1000000 units/mL

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

UniProt accession no.

P17777

shipped in

dry ice

General description

Applications for Rat ESGRO include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.
Product Source: rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.

Application

Applications for Rat ESGRO include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et.al. 1998).

Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et.al. 1998).

At this recommended concentration 106 units of ESGRO is sufficient for 1L of tissue culture media.

Visit www.esgro-lif.com for additional information

Rat LIF is not available for resale.

For research use only; not for use as a diagnostic.

Packaging

1 mL

Quality

The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.

Caution

Note: LIF should not be allowed to come in contact with ruminant or swine.

Physical form

Format: Purified
Purified, sterile liquid. Rat ESGRO is supplied in liquid form as 106 Units ESGRO in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability.



Rat ESGRO is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma.

Storage and Stability

Rat ESGRO is shipped on dry ice. This product is stable for at least 12 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells.



For long term storage it is recommended that Rat ESGRO concentrate be stored at -20°C.



It is recommended that prior to use, Rat ESGRO should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at -20°C. Freeze thawing will reduce potency and is not recommended.

Analysis Note

Specific Activity: Rat ESGRO® is assessed both on murine ES {embryonic stem} cells (MBL-1) and on murine M1 myeloid leukemic cells.A standard of 50 Units is defined as the concentration of Rat ESGRO in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies.106 units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media.Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mLMurine myeloid leukemic, M1 Assay: Specific Activity >108 units/mg

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Generation of germline-competent rat induced pluripotent stem cells.
Hamanaka S. et al.
Testing null
Establishment of rat embryonic stem cell lines that can participate in germline chimerae at high efficiency.
Hirabayashi M. et al.
Molecular Reproduction and Development null
Vadim S Polikov et al.
Journal of neuroscience methods, 190(2), 180-187 (2010-05-18)
A basal lamina gel preparation was incorporated into a modified neuroinflammation cell culture model to test the system as a characterization tool for surface-modified microwires. The extent of gliosis at the surface of gel-coated microwires was quantified in response to
Shinobu Ueda et al.
PloS one, 3(7), e2800-e2800 (2008-07-31)
The rat is a reference animal model for physiological studies and for the analysis of multigenic human diseases such as hypertension, diabetes, neurological disorders, and cancer. The rats have long been used in extensive chemical carcinogenesis studies. Thus, the rat

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