Skip to Content

A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

Organic & biomolecular chemistry (2014-12-17)

S Bag, J-C Tseng, J Rochford

ABSTRACT

Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Sodium borohydride solution, 2.0 M in triethylene glycol dimethyl ether

Sigma-Aldrich

Triethylamine, ≥99.5%

Supelco

Tetrahydrofuran, analytical standard

Sigma-Aldrich

Boron, ≥95% (boron), amorphous powder

Sigma-Aldrich

Boron, crystalline, −60 mesh, 99% trace metals basis

Sigma-Aldrich

Sodium borohydride solution, ~12 wt. % in 14 M NaOH

Sigma-Aldrich

Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free

Sigma-Aldrich

Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

Sigma-Aldrich

2,3-Dichloro-5,6-dicyano-p-benzoquinone, 98%

Sigma-Aldrich

Boron, crystalline, 1 cm, 99.7% trace metals basis

Sigma-Aldrich

Triethylamine, ≥99.5%

Sigma-Aldrich

Triethylamine, ≥99%

Sigma-Aldrich

Triethylamine, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%

Sigma-Aldrich

Tetrahydrofuran, ReagentPlus^®, ≥99.0%, contains 200-400 ppm BHT as inhibitor

Supelco

Tetrahydrofuran, Selectophore^™, ≥99.5%

Sigma-Aldrich

Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder

Sigma-Aldrich

Tetrahydrofuran, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%

Sigma-Aldrich

Tetrahydrofuran, ACS reagent, ≥99.0%, contains 200-400 ppm BHT as inhibitor

Sigma-Aldrich

Tetrahydrofuran, inhibitor-free, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Tetrahydrofuran, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%

Supelco

Tetrahydrofuran, Pharmaceutical Secondary Standard; Certified Reference Material

Boron, monofilament, 50m, diameter 0.2mm

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Phorbol 12-myristate 13-acetate, synthetic, ≥98.0% (TLC)

Sigma-Aldrich

VenPure^® SF, powder

Boron, monofilament, 100m, diameter 0.1mm

Supelco

Triethylamine, analytical standard

Boron, monofilament, 10m, diameter 0.1mm