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  • Evaluation of the cell cytotoxicity and DNA/BSA binding and cleavage activity of some dioxidovanadium(V) complexes containing aroylhydrazones.

Evaluation of the cell cytotoxicity and DNA/BSA binding and cleavage activity of some dioxidovanadium(V) complexes containing aroylhydrazones.

Journal of inorganic biochemistry (2015-01-13)
Subhashree P Dash, Alok K Panda, Sagarika Pasayat, Sudarshana Majumder, Ashis Biswas, Werner Kaminsky, Subhadip Mukhopadhyay, Sujit K Bhutia, Rupam Dinda
ABSTRACT

Three dioxidovanadium(V) complexes [VO2L(1-3)] (1-3) [HL(1)=1-napthoyl hydrazone of 2-acetyl pyridine, HL(2)=2-furoyl hydrazone of 2-acetyl pyridine and H2L(3)=isonicotinoyl hydrazone of 2-hydroxy benzaldehyde] have been reported. All the complexes were characterized by various spectroscopy (IR, UV-visible and NMR) and the molecular structures of 1 and 2 were characterized by single crystal X-ray diffraction technique. Structural report established five-coordinate geometries, distorted toward square pyramidal for each of 1 and 2, based on a tridentate -O,N,N coordinating anion and two oxido-O atoms. The experimental results show that the complexes interact with calf-thymus DNA (CT-DNA) possibly by a groove binding mode, with binding constants of ~10(5)M(-1). All complexes show good photo-induced cleavage of pUC19 supercoiled plasmid DNA with complex 1 showing the highest photo-induced DNA cleavage activity of ~68%. 1-3 also exhibit moderate binding affinity in the range of 10(3)-10(4)M(-1) towards bovine serum albumin (BSA), while all the complexes show good photo-induced BSA cleavage activity. Moreover the antiproliferative activity of all these complexes was studied, which reveal all compounds are significantly cytotoxic towards the HeLa cell line.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
2-Acetylpyridine, ≥99%, FG
Sigma-Aldrich
Salicylaldehyde, redist., ≥99.0% (GC)
Supelco
Salicylaldehyde, analytical standard
Sigma-Aldrich
4′,6-Diamidino-2-phenylindole dihydrochloride, suitable for fluorescence, BioReagent, ≥95.0% (HPLC)
Sigma-Aldrich
Salicylaldehyde, reagent grade, 98%
Sigma-Aldrich
2-Acetylpyridine, ≥99%
Sigma-Aldrich
Salicylaldehyde, ≥98%, FG
Sigma-Aldrich
4′,6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence
USP
2-Acetylpyridine, United States Pharmacopeia (USP) Reference Standard