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  • Comparison between manual aspiration via polyethylene tubing and aspiration via a suction pump with a suction trap connection for performing bronchoalveolar lavage in healthy dogs.

Comparison between manual aspiration via polyethylene tubing and aspiration via a suction pump with a suction trap connection for performing bronchoalveolar lavage in healthy dogs.

American journal of veterinary research (2013-03-28)
Katharine S Woods, Alice M N Defarges, Anthony C G Abrams-Ogg, Howard Dobson, Laurent Viel, Brigitte A Brisson, Dorothee Bienzle
ABSTRACT

To compare the diagnostic quality of bronchoalveolar lavage (BAL) fluid acquired from healthy dogs by manual aspiration via polyethylene tubing (MAPT) and via suction pump aspiration (SPA) with a suction trap connection. 12 healthy adult Beagles. BAL was performed with bronchoscopic guidance in anesthetized dogs. The MAPT was performed with a 35-mL syringe attached to polyethylene tubing wedged in a bronchus via the bronchoscope's biopsy channel. The SPA was performed with 5 kPa of negative pressure applied to the bronchoscope's suction valve via a suction trap. The MAPT and SPA techniques were performed in randomized order on opposite caudal lung lobes of each dog. Two 1 mL/kg lavages were performed per site. Samples of BAL fluid were analyzed on the basis of a semiquantitative quality scale, percentage of retrieved fluid, and total nucleated and differential cell counts. Results were compared with Wilcoxon signed rank tests. Percentage of BAL fluid retrieved (median difference, 16.2%), surfactant score (median difference, 1), and neutrophil count (median difference, 74 cells/μL) were significantly higher for SPA than for MAPT. A higher BAL fluid epithelial cell score was obtained via MAPT, compared with that for samples obtained via SPA (median difference, 1). Results indicated that in healthy dogs, SPA provided a higher percentage of BAL fluid retrieval than did MAPT. The SPA technique may improve the rate of diagnostic success for BAL in dogs, compared with that for MAPT. Further evaluation of these aspiration techniques in dogs with respiratory tract disease is required.

MATERIALS
Product Number
Brand
Product Description

Polyethylene (LDPE), ERM®, certified reference material
Supelco
Polyethylene, analytical standard, for GPC, 2,000
Sigma-Aldrich
Polyethylene, Ultra-high molecular weight, surface-modified, powder, 34-50 μm particle size
Sigma-Aldrich
Polyethylene, Linear low density, melt index 1.0 g/10 min (190°C/2.16kg)
Sigma-Aldrich
Polyethylene, Ultra-high molecular weight, surface-modified, powder, 125 μm avg. part. size
Sigma-Aldrich
Polyethylene, High density, melt index 12 g/10 min (190 °C/2.16kg)
Sigma-Aldrich
Polyethylene, Ultra-high molecular weight, average Mw 3,000,000-6,000,000
Sigma-Aldrich
Polyethylene, High density, melt index 2.2 g/10 min (190 °C/2.16kg)
Sigma-Aldrich
Polyethylene, Medium density
Sigma-Aldrich
Polyethylene, low density, melt index 25 g/10 min (190°C/2.16kg)
Sigma-Aldrich
Polyethylene, average Mw ~4,000 by GPC, average Mn ~1,700 by GPC