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Key Documents

C-41212

Sigma-Aldrich

DetachKit 2

3 x 125 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

packaging

pkg of 3 × 125 ml

technique(s)

cell culture | mammalian: suitable

shipped in

cooler

storage temp.

−20°C

General description

DetachKit 2-125

Application

The DetachKit 2 was developed for gentle and effective detachment of adherent primary human cells in culture and is especially valuable for more sensitive cell types. Each batch is tested on primary human cells.

Components

Each DetachKit 2 consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit 2 comes with a Lower Trypsin/EDTA ratio of 0.025%/0.01%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

Quality

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

Subculture Routine

Click here for more information.

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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