A4540

Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat

IgG fraction of antiserum, saline suspension

• Synonym(s): Anti-Mouse IgM, Goat Anti-Mouse IgM
• MDL number: MFCD00145913
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• conjugate: agarose conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: saline suspension
• species reactivity: mouse
• extent of labeling: ≥5 mg per mL
• technique(s): Ouchterlony double diffusion: suitable
• capacity: 0.4 mg binding capacity
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

IgM exists as a pentamer and is secreted by peritoneal B cells. IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat anti-mouse IgM (μ-chain specific)-agarose antibody is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM myeloma proteins.

Application

Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat has been used in agarose binding assay in primary splenic cells and in immunoprecipitation of soluble lens protein and myelogenous leukemia cell lines.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Goat anti-mouse IgM (μ-chain specific)-agarose antibody has been used for immunoprecipitation and ChIP assays. The product can also be used for Ouchterlony double diffusion applications.

To isolate chimeric proteins that were fused with the IgM heavy chain, CHO supernatant was ran over a column packed with agarose-linked goat anti-mouse IgM mu chain specific.

Biochem/physiol Actions

IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM linked agarose resin have been tested for efficient conjugate binding.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable