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Sigma-Aldrich

pETBlue-2 DNA (uncut) - Novagen

Novagen′s pETBlue-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes.

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About This Item

UNSPSC Code:
41106610
NACRES:
NA.51

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

General description

The pETBlue-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag and His•Tag sequences. The sequence is numbered from the first base of the T7 promoter sequence.

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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