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553525

Sigma-Aldrich

RAD51 Inhibitor, B02

The RAD51 Inhibitor, B02 controls the biological activity of RAD51. This small molecule/inhibitor is primarily used for Cell Structure applications.

Synonym(s):

RAD51 Inhibitor, B02, ( E)-3-Benzyl-2-(2-(pyridin-3-yl)vinyl)quinazolin-4(3H)-one

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About This Item

Empirical Formula (Hill Notation):
C22H17N3O
CAS Number:
1290541-46-6
Molecular Weight:
339.39
MDL number:
MFCD03294274
UNSPSC Code:
12352200
NACRES:
NA.28

Quality Level

100

Assay

≥98% (HPLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

light yellow

solubility

DMSO: 50 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C22H17N3O/c26-22-19-10-4-5-11-20(19)24-21(13-12-17-9-6-14-23-15-17)25(22)16-18-7-2-1-3-8-18/h1-15H,16H2/b13-12+

InChI key

GEKDQXSPTHHANP-OUKQBFOZSA-N

General description

A cell-permeable pyridinylvinyl-quinazolinone compound that is shown to specifically inhibit human RAD51 (IC50 = 27.4 µM). Does not affect RecA even at much higher concentration (~250 µM). Directly interacts with RAD51 (Kd = 5.6 µM), and disrupts its binding to DNA and nucleoprotein filament formation. Blocks double-strand break-induced homologous recombination and enhances sensitivity of cells to Cisplatin (Cat. No. 232120) and Mitomycin C (Cat. No. 47589), (Cat. No. 475820). Diminishes co-aggregate formation between RAD51-ssDNA filament and dsDNA. RAD51-Stimulatory Compound-1, RS-1 (Cat. No. 553510) is also available.

Packaging

Packaged under inert gas

Warning

Toxicity: Regulatory Review (Z)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Huang, F., et al. 2012. J. Med. Chem.55, 3031.
Huang, F., et al. 2011. ACS Chem. Biol.6, 628.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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