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Key Documents

08-110

Sigma-Aldrich

ECL Cell Attachment Matrix

Synonym(s):

ECL Matrix

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

biological source

mouse

Quality Level

100

form

liquid

manufacturer/tradename

Upstate®

technique(s)

cell culture | mammalian: suitable

input

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

ECL Cell Attachment Matrix from Engelbreth-Holm-Swarm (EHS) mouse tumor.

Application

Cell culture attachment factor

Quality

Routinely evaluated in a Neurite Outgrowth Assay using PC12 rat pheochromocytoma cells.

Physical form

5 mg in 5 ml of 0.05 M Tris-HCl, pH 7.4, 0.15 M NaCl. Protein determined by the Bradford dye binding assay using gamma globulin as the standard. Frozen Solution.

Storage and Stability

Stable for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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F B Gertler et al.
Cell, 87(2), 227-239 (1996-10-18)
Drosophila Enabled is required for proper formation of axonal structures and is genetically implicated in signaling pathways mediated by Drosophila AbI. We have identified two murine proteins, Mena and Evl, that are highly related to Enabled as well as VASP
B B Friday et al.
The Journal of cell biology, 149(3), 657-666 (2000-05-03)
Differentiation of skeletal muscle myoblasts follows an ordered sequence of events: commitment, cell cycle withdrawal, phenotypic differentiation, and finally cell fusion to form multinucleated myotubes. The molecular signaling pathways that regulate the progression are not well understood. Here we investigate
Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in vitro.
Hadley, M A, et al.
The Journal of cell biology, 101, 1511-1522 (1985)
Basement membrane complexes with biological activity.
Kleinman, H K, et al.
Biochemistry, 25, 312-318 (1986)
Alteration of Ca2+ dependence of neurotransmitter release by disruption of Ca2+ channel/syntaxin interaction
Rettig, J., et al
The Journal of Neuroscience, 17, 6647-6656 (1997)
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Protocols

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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