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Merck

On-line 2D-LC-ESI/MS/MS determination of rifaximin in rat serum.

Biomedical chromatography : BMC (2009-05-16)
R Nageswara Rao, R Mastan Vali, Dhananjay D Shinde
PMID19444797
ABSTRACT

A highly sensitive and selective on-line two-dimensional reversed-phase liquid chromatography/electrospray ionization-tandem mass spectrometry (2D-LC-ESI/MS/MS) method was developed and validated to determine rifaximin in rat serum by direct injection. The 2D-LC-ESI/MS/MS system consisted of a restricted access media column for trapping proteins as the first dimension and a Waters C(18 )column as second dimension using 0.1% aqueous acetic acid:acetonitrile as mobile phase in a gradient elution mode. Rifampacin was used as an internal standard. The linear dynamic range was 0.5-10 ng/mL (r(2) > 0.998). Acceptable precision and accuracy were obtained over the calibration range. The assay was successfully used in analysis of rat serum to support pharmacokinetic studies.

MATERIALS
Product Number
Brand
Product Description
505161
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
569232-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4.6 mm
504947
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
504955
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
569229-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 2.1 mm
504971
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
505137
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea
505188
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm, pkg of 2 ea
504971-30
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm
59576-U
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 3 mm, pkg of 2 ea
50494721
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm
50495521
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
59575-U
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 3 mm
569222-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4 mm
569224-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
569223-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
569220-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
505129
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm
504971-40
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
504955-40
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
504955-30
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 3 mm
569226-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
569231-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4 mm