Skip to Content
Merck
All Photos(1)

Key Documents

G005697

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si (25 cm Column)

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] hexane; [B] ethyl acetate
gradient: 10 to 30% B in 10 min; held at 30% B for 2 min
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 290 nm
injection: 10 μL

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    (±)-α-Tocopherol, analytical standard
    Menadione (K3), analytical standard
    (+)-γ-Tocopherol, analytical standard
    Cholecalciferol (D3), analytical standard

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service