SHM01
MISSION® ExpressMag® Super Magnetic Kit
Increases transduction efficiency
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About This Item
Recommended Products
product line
MISSION®
shelf life
≥2 yr at ~4 °C
storage temp.
2-8°C
General description
To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols
www.sigmaaldrich.com/mission-protocols
Application
MISSION® ExpressMag® Super Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes. The kit has been documented to enhance MISSION® lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag™ system.
Caution
Do not autoclave! Clean surface of magnet with 70% isopropyl alcohol.
Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.
Legal Information
ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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