S8633
Sphingomyelinase from Staphylococcus aureus
buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)
Synonym(s):
Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32
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form
buffered aqueous glycerol solution
Quality Level
specific activity
100-300 units/mg protein (Lowry)
storage temp.
2-8°C
Application
Sphingomyelinase from Staphylococcus aureus has been used to:
- induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
- determine the concentration of sphingomyelin from serum samples
- enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis
Biochem/physiol Actions
Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Unit Definition
One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
Physical form
Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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In this study, we determined whether minocycline may protect rat cortical cultures against neurotoxicity induced by sphingomyelinase/ceramide and explored the underlying mechanisms. We found that minocycline exerted strong neuroprotective effects against toxicity induced by bacterial sphingomyelinase and synthetic C2 ceramide.
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