The IL-10 standard is prepared by diluting 5 uL with 728.3 uL of Assay Diluent A or Assay Diluent B. The formulations of both diluents are proprietary and may not be shared. Please see the link below for the sample Certificate of Analysis to review the protocol. Note the standard preparation instructions on page 2: https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0477_1013J0430.pdf
RAB0477
Mouse Tumor Necrosis Factor α ELISA Kit
for serum, plasma and cell culture supernatant
Synonym(s):
Cachectin, TNF-alpha, Tumor necrosis factor alpha
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
Pricing and availability is not currently available.
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species reactivity
mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type serum
sample type cell culture supernatant(s)
sample type plasma
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Tnf(21926)
General description
The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.
Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.[1]
Immunogen
Recombinant Mouse TNF-α
Application
Biochem/physiol Actions
Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.[6]
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Kit Components Also Available Separately
Product No.
Description
SDS
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
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How to prepare lyophilized reagents from ELISA kits for IL10 elisa sigma RAB 0244-1KT and on standard dilution: 5microl + 728.3 microl BUT WHAT IS 728.3 microl
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