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69452-M

Sigma-Aldrich

HMS174 Competent Cells - Novagen

Escherichia coli, rod shaped

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About This Item

UNSPSC Code:
41106202
NACRES:
NA.81

product name

HMS174 Competent Cells - Novagen, HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

biological source

Escherichia coli

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

transformation efficiency: >5×106 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

Genotype: F-recA1 hsdR(rK12- mK12+) (Rif R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
HMS174 strains provide the recA mutation in a K-12 background. Like BLR, these strains may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlHMS174 Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

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Robert L Garcea et al.
NPJ vaccines, 5(1), 45-45 (2020-06-13)
Cold-chain requirements affect worldwide distribution of many vaccines. In addition, vaccines requiring multiple doses impose logistical and financial burdens, as well as patient compliance barriers. To address such limitations, we have developed new technologies to prepare thermostable, single-shot, prime-boost microparticle

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