Skip to Content
Merck
  • Microfluidic aqueous two-phase extraction of bisphenol A using ionic liquid for high-performance liquid chromatography analysis.

Microfluidic aqueous two-phase extraction of bisphenol A using ionic liquid for high-performance liquid chromatography analysis.

Analytical and bioanalytical chemistry (2015-03-23)
Linlin Qi, Yunhua Wang, Yajie Li, Guoxia Zheng, Changping Li, Hongzhen Su
ABSTRACT

An aqueous two-phase microfluidics (ATPM) method suitable for selective extraction of bisphenol A (BPA) in aqueous samples was developed, and a functional ionic liquid of N, N, N-trioctyl ammonium propionate (TOAP) was specially employed for the formation of a parallel flow system. Based on the analytical model, we optimized the chip design into branch-connection length pattern to achieve maximum extraction efficiency (φ max) and ensure phase separation. In combining the design flexibility and ideal reaction activity of extractant (TOAP), the developed ATPM enabled a selective and effective extraction of BPA (φ max of 95% within 2 s) from phenol derivatives. Meanwhile, the total operation time and ionic liquid consumption of the microfluidic extraction were only 2.5 min and 5 μl, respectively. The ATPM can be run at normal pH and room temperature and showed no interferences from components found in tap or beach water. To be noted, this specific extraction system was applied in real water samples; the recoveries of standard addition for all water samples spiked with BPA were from 96 to 110%. Finally, successful reuse of the chip was also realized. In all cases, the developed microfluidic chip was proven to be useful as an effective and low consumption approach in extracting BPA and should be expanded as a "green" preparative method for high-performance liquid chromatography (HPLC) analysis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Resorcinol, ACS reagent, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Resorcinol, ReagentPlus®, 99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Resorcinol, meets analytical specification of Ph. Eur., BP, 98.5-100.5% (calc. to the dried substance)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Resorcinol, ≥98%, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Resorcinol, BioXtra, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Resorcinol, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
USP
Resorcinol, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
Resorcinol, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
SKU
Pack Size
Availability
Price
Quantity
Hymecromone impurity A, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, unstabilized, ReagentPlus®, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Phenol solution, 100 μg/mL in acetonitrile, PESTANAL®, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, TE-saturated, ~73% (T)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, ≥96.0% (calc. on dry substance, T)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Phenol, PESTANAL®, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, contains hypophosphorous as stabilizer, loose crystals, ACS reagent, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Supelco
Phenol solution, certified reference material, 500 μg/mL in methanol
SKU
Pack Size
Availability
Price
Quantity
Supelco
Phenol solution, 5000 μg/mL in methanol, certified reference material
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Liquified Phenol, ≥89.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol solution, BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol solution, BioReagent, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, puriss., ≥99.5% (GC), meets analytical specification of Ph. Eur., BP, USP, crystalline (detached)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Phenol, BioXtra, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Phenol, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
USP
Phenol, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity