HPLC Discovery HS C18

Discovery HS C18 HPLC Columns, 120 Å;
3 µm, 5 µm and 10 µm particle sizes available

• Specifically developed for pharmaceutical analysis and purification
• Ideal for LC/MS applications - No detectable bleed (as independently tested)
• Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life
• Scalable from analytical to prep

NO DETECTABLE LC/MS BLEED

Discovery HS C18

No TIC Baseline rise.
Essentially no m/z peaks generated from column ("x" indicates peaks also found in blank run)

Column: Discovery HS C18 and Conventional C18, 5cm x 4.6mm, 3µm;
0.1% TFA; Flow Rate: 1mL/min; Temp: ambient, esi+ ion.

Competitor C18

TIC Baseline rise can interfere with sensitivity, and quantitation measurements. Bleed m/z peaks could coincidently interfere with "real" peaks.

Mobile Phase: 15 min. gradient from 0-100% MeOH in

(work conducted by Dr. Shane Needham, Alturas Analytics, Moscow, ID)

LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS C18

Column: Discovery HS C18, 15 cm x 4.6 mm, 5 µm
Mobile Phase: 80% 10 mM ammonium acetate, pH 6.8 : 20% MeOH
Flow Rate: 1 mL/min
Temp: 35 °C
Detection: 254 nm;
Injection: 1 µL

1. Uracil (8 µg/mL)
2. Procainamide (30 µg/mL)
3. Sorbic acid (4 µg/mL)
4. Pyridine (100 µg/mL)
5. Caffeine (40 µg/mL)
6. Phenol (240 µg/mL)

GREATER HYDROPHOBIC RETENTION COMPARED TO OTHER POPULAR C18 PHASES

Columns: 7.5 cm x 4.6 mm, 3 µm/3.5 µm
Mobile Phase: 35:65 - H₂O:ACN
Flow Rate: 2 mL/min; Detection: 254 nm

Ordering Information:

* 569275-U (Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules)