Skip to Content
Merck
All Photos(3)

Documents

SAB4500349

Sigma-Aldrich

Anti-Histone H3 (Acetyl-Lys18) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

H3/a, H3/c, H3/d, H3/f, H3/h

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 15 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P68431

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys18)

Gene Information

human ... HIST1H3A(8350)

General description

Anti-Histone H3 (Acetyl-Lys18) Antibody detects endogenous levels of total Histone H3 (Acetyl-Lys18) protein.
Histone H3 is located on chromosome 6p22. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18.

Immunogen Range: 1-50

Application

Anti-Histone H3 (Acetyl-Lys18) antibody has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Histone proteins are basic building blocks of chromatin. Mutations in Histone H3 results in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that “drive” chromatin condensation.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Inhibition of different histone acetyltransferases (HATs) uncovers transcription-dependent and-independent acetylation-mediated mechanisms in memory formation.
Merschbaecher K, et al.
Learning & Memory, 23(2), 83-89 (2016)
Katja Merschbaecher et al.
Learning & memory (Cold Spring Harbor, N.Y.), 23(2), 83-89 (2016-01-17)
Acetylation of histones changes the efficiency of the transcription processes and thus contributes to the formation of long-term memory (LTM). In our comparative study, we used two inhibitors to characterize the contribution of different histone acetyl transferases (HATs) to appetitive
The program for processing newly synthesized histones H3.1 and H4.
Campos EI
Nature Structural and Molecular Biology, 17(11), 1343-1351 (2010)
Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34(3), 113-119 (2017)
Signaling to chromatin through histone modifications.
Cheung P, et al.
Cell, 103(2), 263-271 (2000)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service