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MAK193

Sigma-Aldrich

Citrate Synthase Activity Assay Kit

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

Citrate Synthase Test Kit

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

Gene Information

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

General description

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.[1]

Application

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.[2]

Suitability

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.

Kit Components Only

Product No.
Description

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Questions

  1. Is the sensitivity information for the CS0720 (Citrate Synthase Assay Kit) available?

    1 answer
    1. The lower concentration tested for the product CS0720 (Citrate Synthase Assay Kit) is 1u/µgP

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 0
    1 out of 5 stars.

    This actually measures total deacetylation activity, not specific to CS

    The mechanism for the assay kit is the deacetylation of Acetyl-CoA to CoA. There are multiple enzymes can do this, not just CS. This assay kit doesn't have a good control for the background non-CS-related deacetylation step, so it is not the right product to measure CS activity. CS0720 is the CS specific assay kit to buy.

    Helpful?

    1. Response from MilliporeSigma:

      MAK193 has been discontinued, CS0720 is its recommended replacement. CS0720 includes a positive control (which MAK193 lacked). CS0720 provides the Acetyl Coenzyme A and the Oxaloacetic acid needed for Citrate Synthase to produce Citrate.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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