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FCMAB291F

Sigma-Aldrich

Milli-Mark®Anti-Cytokeratin 5 Antibody, 6-FITC, clone D5/16B4

clone D5/16B4, Milli-Mark®, from mouse

Synonym(s):

58 kDa cytokeratin, epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5, keratin 5 (epidermolysis bullosa simplex,Dowling-Meara/Kobner/Weber-Cockayne types), keratin, type II cytoskeletal 5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

D5/16B4, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

NP_000415.2

UniProt accession no.

P13647

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KRT5(3852)

General description

The cytokeratins are a family of intermediate filaments found in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or more acoring to molecular weight and isoelectric point (Quinlan et al. 1985). Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen′s disease. It does not react with pulmonary adenocarcinoma.

Specificity

Antibody recognizes Human Cytokeratin.

Immunogen

Purified cytokeratin 5 (Wild, 1986) (Mischke, 1987).

Application

Milli-MarkAnti-Cytokeratin 5 Antibody, 6-FITC, clone D5/16B4 is an antibody against Cytokeratin 5 for use in FC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Evaluated by flow cytometry using A431 cells

Target description

The calculated for Cytokeratin is 62 kDa, however, different isoforms state Cytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).

Physical form

Protein A purified
Purified mouse monoclonal IgG1 conjugated to FITC in PBS buffer with 15mg/mL BSA and sodium azide at 0.1%.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Analysis Note

Control
A431 cells

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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