16-101
Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
clone 4G10®, Upstate®, from mouse
Synonym(s):
4G10 Phosphotyrosine Antibody, Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4G10®, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PID1(55022)
General description
Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Specificity
Recognizes tyrosine-phosphorylated proteins from all species.
Immunogen
Phosphotyramine-KLH.
Application
Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Suitable for immunoprecipitation and affinity purification.
Suitable for immunoprecipitation and affinity purification.
Features and Benefits
Format: Gel Immobilized
Quality
Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Target description
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physical form
1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon as a preservative, for a total volume of 2 mL. Liquid suspension.
Protein A purified
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Phosphorylation site dynamics of early T-cell receptor signaling.
Chylek, LA; Akimov, V; Dengjel, J; Rigbolt, KT; Hu, B; Hlavacek, WS; Blagoev, B
Testing null
Heparin binding and oligomerization of hepatocyte growth factor/scatter factor isoforms. Heparan sulfate glycosaminoglycan requirement for Met binding and signaling.
Sakata, H, et al.
The Journal of Biological Chemistry, 272, 9457-9463 (1997)
Growth hormone induction of hepatic serine protease inhibitor 2.1 transcription is mediated by a Stat5-related factor binding synergistically to two gamma-activated sites
Bergad, P. L., et al
The Journal of Biological Chemistry, 270, 24903-24910 (1995)
Endogenous reactive oxygen intermediates activate tyrosine kinases in human neutrophils
Brumell, J. H., et al
The Journal of Biological Chemistry, 271, 1455-1461 (1996)
TNF-alpha induces tyrosine phosphorylation of mitogen-activated protein kinase in adherent human neutrophils.
Rafiee, P, et al.
Journal of immunology (Baltimore, Md. : 1950), 154, 4785-4792 (1995)
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