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  • Direct effect of 2-palmitoyl glycerol on promotion of gamma aminobutyric acid synthesis in normal human fetal-derived astrocytes.

Direct effect of 2-palmitoyl glycerol on promotion of gamma aminobutyric acid synthesis in normal human fetal-derived astrocytes.

FEBS open bio (2023-05-18)
Misato Tsuboi, Yoshitaka Nakamura, Hiroshi Sakuma
ABSTRACT

Breast milk contains constituents, such as 1,3-dioleoyl-2-palmitoylglycerol (OPO), that are beneficial for infants. Herein, we hypothesized that 2-palmitoyl glycerol (2-PG), a derivative of OPO, is advantageous to infants' development. Gamma aminobutyric acid (GABA) is a major neurotransmitter involved in neural development. Although GABA is generally known to be produced in neurons, astrocytes can also produce it in immature brains. In this study, we used expression analysis techniques to show that 2-PG upregulates the mRNA and protein expression of glutamate decarboxylases (GAD1 and GAD2) in normal human fetal-derived astrocytes. Our data suggest that 2-PG promotes GABA synthesis in astrocytes, which may contribute to brain development because GABA is involved in neural development in the developing brain. This may help to elucidate the mechanism by which breast milk affects infant brain development.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-VGAT antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL2793, purified immunoglobulin, buffered aqueous glycerol solution