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RAB0105

Sigma-Aldrich

Human Cystatin C ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

Cystatin-3, Cystatin-C

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type urine
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 300 pg/mL
standard curve range: 0.313-20 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CST3(1471)

Related Categories

General description

The Human Cystatin C ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human cystatin C in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CystatinC

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Cystatin C (CysC) is released by all nucleated cells. Abnormal thyroid function, immunosuppressive therapy and systemic inflammation alter the levels of CysC. Mutation in the gene encoding CysC causes hereditary cystatin C amyloid angiopathy, which is a consequence of amyloid deposits containing the CysC variant in the brain.
Cystatin-C precursor (CST3) is an inhibitor of lysosomal cysteine proteases and several cathepsins like cathepsin S, a lysosomal enzyme. It has been shown that CST3 is a marker of glomerular filtration and diabetic kidney disease. In the presence of pathological conditions, it forms amyloid fibrils in brain arteries. Mutations in the gene encoding CST3 have been shown to be associated with a form of amyloidosis and age-related macular degeneration.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The pentapeptide LQVVR plays a pivotal role in human cystatin C fibrillization.
Tsiolaki PL, Hamodrakas SJ, Iconomidou VA.
Febs Letters, 589(1), 159-164 (2015)
Stroke in Icelandic patients with hereditary amyloid angiopathy is related to a mutation in the cystatin C gene, an inhibitor of cysteine proteases.
Levy E et.al.
The Journal of Experimental Medicine, 169(5), 1771-1778 (1989)
CST3 genotype associated with exudative age related macular degeneration.
Zurdel J et.al.
The British Journal of Ophthalmology, 86(2), 214-219 (2002)
Yuanyuan Pei et al.
European journal of medical research, 27(1), 39-39 (2022-03-12)
Acute kidney injury (AKI) is a common and critical complication of sepsis, and is associated with unacceptable morbidity and mortality. Current diagnostic criteria for AKI was insensitive for early detection. Novel biomarkers including cystatin C, kidney injury molecule-1 (KIM-1), neutrophil
Handbook of Neurochemistry and Molecular Neurobiology: Neural Protein Metabolism and Function, 254-254 (2009)
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