Anaerobic Culture Methods with the Anaerocult® System
Creating Anaerobic, Microaerobic, and Capneic Growth Conditions for Microbiology
In many industries, including food, beverage, or clinical microbiology, it is often necessary to create an anaerobic environment to facilitate the growth of anaerobes, microaerophiles, and capnophiles such as Clostridium, Campylobacter, or Lactobacillus. Creating such organisms requires the creation and maintenance of appropriate conditions, including the gaseous environment.
Our Anaerocult® systems produce the anaerobic, microaerobic, and capneic growth conditions that are required for anaerobiosis.
How Our Anaerobic Incubation Systems Work?
The Anaerocult® system contains sachets made up of highly absorptive and gas permeable paper. The Anaerocult® reagent mixture react with water to chemically bind to all or part of the atmospheric oxygen (O2) in a given volume of air (anaerobic jar or incubation bag), to produce carbon dioxide (CO2) gas, creating an oxygen-free (anaerobic) or oxygen-deficient (microaerobic/capneic) atmosphere. We have a range of cost-efficient Anaerocult® products that offers the laboratory with benefits of user defined environments to fit your microbiology needs. The products are catalyst-free and don't create high temperatures which can negatively impact the growth of microorganisms.
Anaerocult® System for Cultivation of Microaerophilic and Capnophilic Microorganisms
For the cultivation of capnophilic and microaerophilic microorganisms that thrive in conditions where concentrations of carbon dioxide are greater than in the earth’s atmosphere. To generate CO2-enriched cultivating conditions we provide the Anaerocult® C and Anaerocult® C Mini systems.
Anaerocult® C System
Our Anaerocult® C system is used specifically for the incubation of microaerophiles and capnophiles. The special formulation of the reagent mixture guarantees that the cultivation jar reaches gas concentrations of 8-10% vol. CO2 and 5-6% vol. O2. At an incubation temperature of 42 °C, the temperature in the jar does not increase further. This is because, unlike systems using a catalyst, the amount of energy released is low, so the microorganisms are not harmed by the heat.
Anaerocult® C Mini Sachet for Incubation of One or Two Petri Dishes
Anaerocult® C Mini system is a gas generation system for incubation where one or two petri dishes can be placed in the special incubation bag. The petri plate can be two different culture media plates inoculated with one sample or two plates with the same medium inoculated with two different samples. The Anaerocult® C Mini sachet is moistened with 3 mL of water and placed in the special incubation bag with the petri dishes. It contains chemicals that bind a precisely determined amount of oxygen and release a defined quantity of CO2. This creates just the right atmospheric conditions in which microaerophilic or capnophilic microorganisms can grow.
Anaerocult® System for Cultivation of Anaerobic Microorganisms
For the cultivation of facultative or obligate anaerobic microorganisms, we offer the Anaerocult® incubation system, comprising Anaerocult® A, Anaerocult® A Mini, Anaerocult® P, and Anaerotest® test strips. Anaerobes are clinically important pathogenic agents responsible for infectious diseases and the spoilage of food and beverages. Our Anaerocult® products cover a wide range of requirements for cultivating anaerobic microorganisms for clinical microbiology, or the food and beverage industries.
Anaerocult® A System for Incubation of Up to 12 Petri Dishes
The Anaerocult® A system is used for the anaerobic incubation of up to 12 petri dishes in an anaerobic jar or bag. This reagent contains components that create an oxygen-free (anaerobic) environment in the jar and simultaneously release CO2. The contained oxygen does not react with hydrogen to form H2O but is chemically bound to the finely distributed iron powder in the reagent mixture.
Anaerocult® A Mini Sachet for Incubation of One to Four Petri Dishes
The Anaerocult® A mini system is used for the anaerobic incubation of one to four petri dishes. The Anaerocult® A mini sachet is moistened with water and placed in a special incubation bag along with the petri dishes and an Anaerotest® test strip to monitor the absence of oxygen. The sachet contains chemical components that bind to the oxygen quickly and completely, creating an oxygen-free and CO2-rich atmosphere within the bag.
Anaerocult® P Sachet for Incubation of Single Petri Dishes
Anaerocult® P system is used for anaerobic incubation of single petri dishes. The Anaerocult® P sachet is moistened with water and placed in a special incubation bag, which is subsequently sealed. It contains components that chemically bind to the oxygen quickly and completely, creating an oxygen-free atmosphere rich in CO2. The transparent incubation bag allows anaerobic growth in the closed anaerobic system that can be evaluated visually at any given time. Each petri dish can be incubated immediately after receiving the sample, so the Anaerocult® P sachet is ideal for both urgent cases and for the cultivation of oxygen-sensitive microorganisms. Incubation of individual petri dishes permits each one to be cultivated separately over time and at the required temperature. It also prevents interactions between the microorganisms on different plates.
Anaerotest® Test Strips
The Anaerotest® test strip is used to check the anaerobic atmosphere condition in sealed containers or special incubation bags. The reaction zone of the test strip contains a dye that is blue (oxidized state) if in an air-filled (i.e., aerobic) environment. An anaerobic environment initiates the chemical reduction of the dye, which then changes its color to white. The reaction is reversible so that when oxygen is re-introduced, the reaction zone turns blue again. The strips do not change color when used with the Anaerocult® C and C Mini kits due to the residual oxygen content in the atmosphere of the bags.
Anaeroclip® Sealing Device
The Anaeroclip® is a simple mechanical clip used to seal the Anaerocult® A Mini, P, and C Mini incubation bags. The Anaeroclip® closure clips enable users to seal the bags without a special foil sealing instrument and can be used several times.
Anaerobic Jar and Plate Basket
The anaerobic jar consists of two functional parts: a lid and a base. The jar is easy to use, convenient, and time-saving since there is no need to insert or refill catalysts or use manometers or valves. The jar is manufactured using high-quality materials, ensuring that it is leak-proof and that the lid always fits properly. The plate basket is designed to conveniently place the plates into the jar and comes with an integrated holding clip for the Anaerotest® strip.
CULTURA Mini-Incubator
The CULTURA mini-incubator, a benchtop system, is an ideal incubator for a small laboratory and can be used to incubate Anaerocult® A mini, C mini, and P. It can also fit 18 dip slides or 12 petri dishes, microbiological samples, blood culture bottles, or identification systems of any kind. The incubator is safe to handle, saves space, easy to clean, and allows various incubation temperatures. It is available as an all-in-one package that includes a mini-incubator, multi-rack, and thermometer.
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