Home Single Molecule Counting (SMC^®) TechnologyWhat Is SMC^® Technology?: An Overview of Ultrasensitive Immunoassay Technology

What Is SMC^® Technology?: An Overview of Ultrasensitive Immunoassay Technology

Curious about how ultrasensitive immunoassay technology can enhance your research? Learn how SMC^® technology is advancing research in immunology, cardiotoxicity, neuroscience, and more. Also, explore the advantages of SMC^® technology and see how researchers are using these high sensitivity assays to advance their discoveries.

Section Overview

What Is SMC^® Technology?
SMC^® Technology Workflow
Ultrasensitive Biomarker Detection Platform
Research Applications
Frequently Cited Advantages of SMC^® Technology
Tips and Tricks for Running SMC^® Assays
Related Webinars and Events
Publications Using SMC^® Technology

What Is SMC^® Technology?

SMC^® technology is an advanced ultrasensitive immunoassay technology that gives researchers the power to detect biomarkers that were previously undetectable down to the femtogram/mL. It provides an indispensable tool in the researcher’s arsenal to help them move novel biology forward, fueling the discovery and development of new therapeutics. SMC^® technology, originally developed by Singulex^®, Inc. in 2004, relies on a basic sandwich immunoassay format utilizing two antibodies specific to the analyte of interest: a capture antibody coated on a plate or magnetic bead and a detection antibody conjugated to a fluorescent protein. Only SMC^® immunoassay technology allows researchers to use both plate-based and bead-based assay designs, thus offering unmatched flexibility in assay design.

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SMC^® Technology Workflow

Scientists familiar with traditional immunoassays, such as ELISAs, know that sensitivity, matrix effects, and dynamic range affect how they make measurements — or if they can make them at all. Combining a traditional immunoassay workflow with patented SMC^® technology enables the detection of low-abundant biomarkers, such as proteins and nucleic acids, with unparalleled sensitivity and accuracy, capturing concentrations down to the femtogram/mL level. Researchers can detect, and monitor changes in, extremely low levels of established disease biomarkers such as cardiac troponin I and cytokines.

The SMC^® technology workflow has 5 steps described below. Distinguishing SMC^® assays from traditional immunoassays, an elution buffer is used to break apart the immunoassay complex once constructed. The eluate containing the fluorescent reporter molecule is transferred to a 384-well plate, thus removing other assay components that contribute to high background fluorescent signal, such as the magnetic beads and antibodies used for analyte capture. The plate is loaded into the SMCxPRO^® instrument, the second-generation SMC^® instrument, where a laser excites the fluorescent-labeled detection antibody as it passes through a narrow interrogation window. Individual photons are captured by an avalanche photodiode and the signal is recorded. This allows for the digital quantification of individual molecules. Analyte concentrations in the unknown samples are calculated using the corresponding standard curve.

The steps in the SMC^® Technology Workflow include:

Sample Prep

Analyte of interest in solution

Capture

Capture on plate or bead

Detect

Antibodies translate each biomarker into a signal

Elute

Complex is chemically broken apart

Quantify

Sample is detected by laser and detection tags are counted

After following a traditional sandwich ELISA workflow, the proprietary SMC^® protocol steps concentrate the signal by disassociating the fluorescent-labeled detection antibody from the sandwich complex. The fluorescent-labeled detection antibody is the signal acquired in the SMCxPRO^® instrument. This results in reproducible signal, and improved quantification of proteins, particularly those at very low abundance. With better precision and sensitivity, researchers can:

Quantify previously undetectable analytes
Better stratify sample populations
Gain insights into novel biological mechanisms
Require fewer data points for critical decision making
Accelerate drug discovery and development
Reduce program costs and improve productivity

Ultrasensitive Biomarker Detection Platform

SMC^® technology offers an ultrasensitive biomarker detection platform with the flexible SMCxPRO^® platform. Table 1 compares running assays on this platform to running traditional ELISAs.

Ancillary Equipment and Kits to Enhance the SMC^® Workflow

To enhance the SMCxPRO^® platform, we also offer ancillary equipment and kits specifically for SMC^® technology, such as:

Research Applications

Ultrasensitive immunoassays can enhance research in a variety of fields because the improved sensitivity allows researchers to dive deeper into their studies. Research applications of SMC^® technology include the areas of:

Frequently Cited Advantages of SMC^® Technology

SMC^® technology provides maximum immunoassay performance while following a workflow very similar to traditional ELISA technology.

Traditional ELISA methodologies demonstrate limitations in sensitivity and dynamic range, typically require high sample volumes, and are susceptible to matrix effects. Combined, these factors reduce the utility of traditional ELISAs for the detection of low-abundant proteins and endogenous biomarker levels in healthy subjects, thus hampering statistical analysis among study groups. By adapting an ELISA workflow, SMC^® technology achieves improved signal-to-noise ratios over traditional immunoassay technologies, thus providing quantification at both low and high levels of expression in one complete system. Digital counting of fluorescent events improves the assay sensitivity and extends the assay dynamic range beyond what can be achieved with traditional immunoassays.

Because SMC^® immunoassay technology can reach fg/mL sensitivity ranges, this platform offers the ability to dilute pre-clinical samples, when only low sample volumes are available.

Users are fully supported by onsite Immunoassay Field Application Scientists and Specialists, as well as dedicated technical support teams.

We understand the SMC^® platform is an important investment for research labs and is committed to ensuring the success of its users. Regardless of the types of assays being used, all SMC^® users are fully supported by onsite Immunoassay Field Application Scientists and Specialists who have experience working with researchers from a broad range of lab types, including academic, government, biotech, pharma, CRO, and regulated labs.

The SMC^® platform is a versatile system that can be used in multiple study types, including biomarker assessment, bioanalytical work such as pharmacokinetic and pharmacodynamic studies, and immunogenicity testing.

Complimenting a menu of off-the-shelf, verified assay kits, our Custom Assay Development and Innovation (CADI) team can be contracted to perform custom assay development and sample testing services at their site in St. Louis, MO, USA. Request more information about our custom services on our CADI page.

SMC^® assays are available in bead-based formats and can be developed in both plate-based and bead-based formats by our CADI team.

The proprietary SMC^® technology allows scientists to measure proteins with increased precision, enabling unparalleled quantification at low and high abundant levels of expression. The flexible SMC^® immunoassay system acquires data from both plate-based assays and bead-based assays, providing a choice of format depending on budget and quantification requirements. Learn more about how we develop custom SMC^® assays in our article on CADI services.

The SMC^® assay read plate is a 384-well plate and the entire plate can be read in less than three hours. This high-throughput platform allows researchers to perform an entire SMC^® assay run from sample prep through data analysis in one day.

SMCxPRO^® system can be integrated with a Hamilton Microlab^® STARlet liquid handing system, increasing assay throughput.

In certain environments, automation of SMC^® immunoassays is desirable so that researchers can focus on other high-value activities to increase overall efficiency. The Hamilton Microlab^® STARlet liquid handling workstation offers a hands-free option providing a robust, reproducible SMC^® workflow eliminating sources of error and variability. Our Custom Assay Development and Innovation (CADI) team uses this technology when applicable to reduce lead time on sample testing projects.

Both data acquisition and analysis are performed within a single software package.

The SMCxPRO^® software package was developed in-house, thus affording full transparency to data processing algorithms. The software is user-friendly and allows end-users to set up the instrument, read the plate, and analyze results quickly and easily. The software enables easy data curation, including manual outlier removal.

For labs operating in a regulated environment, the SMCxPRO^® instrument generates one signal data stream that can be imported into Laboratory Information Management Systems, such as WATSON, or other software. 21 CFR Part 11 compliance features can also be enabled.

Tips and Tricks for Running SMC^® Assays

Have questions about running SMC^® assays? Check out helpful tips and tricks in our guide to high sensitivity biomarker analysis with SMC^® technology.

Related Webinars

Publications Using SMC^® Technology

See how researchers are using SMC^® technology in the publications list below organized by research area.

Neurology

A Critical Appraisal of Blood-Based Biomarkers for Alzheimer’s Disease. Lista S, Mapstone M, Filippo Caraci, Emanuele E, López-Ortiz S, Martín-Hernández J, Triaca V, Imbimbo C, Gabelle A, Mielke MM, et al. 2024 Apr 1. Ageing research reviews (Print).:102290–102290. doi:10.1016/j.arr.2024.102290.
CSF biomarkers of immune activation and Alzheimer’s disease for predicting cognitive impairment risk in the elderly. Shue F, White LJ, Hendrix R, Ulrich J, Henson RL, Knight W, Martens YA, Wang N, Roy B, Starling SC, et al. 2024. Science Advances. 10(14). doi:10.1126/sciadv.adk3674.
Biochemical, Biomarker, and Behavioral Characterization of the GrnR493X Mouse Model of Frontotemporal Dementia. Smith DM, Aggarwal G, Niehoff ML, Jones SA, Banerjee S, Farr SA, Nguyen AD. 2024 May 2. Molecular Neurobiology. doi:10.1007/s12035-024-04190-9.
ACKR3 Antagonism Enhances the Repair of Demyelinated Lesions Through Both Immunomodulatory and Remyelinating Effects. Laetitia Pouzol, Sassi A, Tunis M, Anaïs Zurbach, Nadège Baumlin, Gnerre C, Strasser DS, Marrie J, Enrico Vezzali, Martinic MM. 2024. Neurochemical Research. 49(8):2087–2104. doi:10.1007/s11064-024-04173-1.
Recent Advancements in Biosensors for the Detection and Characterization of Amyloids: A Review. Rashid MH, sen P. 2024. The Protein Journal. 43(4):656–674. doi:10.1007/s10930-024-10205-0.
Molecular Biomarkers of Neurodegenerative Disorders: A Practical Guide to Their Appropriate Use and Interpretation in Clinical Practice. Agnello L, Caterina Maria Gambino, Anna Maria Ciaccio, Masucci A, Vassallo R, Tamburello M, Scazzone C, Bruna Lo Sasso, Ciaccio M. 2024. International journal of molecular sciences. 25(8):4323–4323. doi:10.3390/ijms25084323.
Detection of high levels of mutant huntingtin protein in tear fluid of patients with Huntington’s Disease. Marlies Gijs, Nynke Jorna, Datson N, Beekman C, Dansokho C, Weiss A, Linden D, Mayke Oosterloo. 2024. Investigative Ophthalmology & Visual Science. ARVO Annual Meeting Abstract. 65(7):3322–3322.
Enhancing clinical trials methodology in Huntington’s disease. Rodrigues B. 2024 Jan 28. UCL Discovery.
High levels of mutant huntingtin protein in tear fluid from Huntington’s Disease Gene Expansion Carriers. Gijs M, Jorna N, Datson N, Beekman C, Dansokho C, Weiss A, Linden DEJ, Oosterloo M. 2024 Feb 21. Journal of Movement Disorders. doi:10.14802/jmd.24014.
Lower Plasma Amyloid Beta - 42 Levels Associated With Worse Survival in Patients With Glioma. SEO K, HWANG K, NOH M, PARK J, AHN K-S, SO YOUNG JI, JUNG HO HAN, KIM C-Y. 2023. In Vivo. 38(1):425–430. doi:10.21873/invivo.13455.
Micromotor-based electrochemical immunoassays for reliable determination of amyloid-β (1–42) in Alzheimer’s diagnosed clinical samples. José M. Gordón Pidal, María Moreno-Guzmán, Montero-Calle A, Valverde A, Pingarrón JM, Campuzano S, Calero M, Barderas R, Miguel Ángel López, Escarpa A. 2023. Biosensors and Bioelectronics. 249:115988–115988. doi:10.1016/j.bios.2023.115988.
Anxiety adds the risk of cognitive progression and is associated with axon/synapse degeneration among cognitively unimpaired older adults. Sun L, Li W, Qiu Q, Hu Y, Yang Z, Xiao S. 2023. eBioMedicine. 94:104703. doi:10.1016/j.ebiom.2023.104703.
Receptor-Independent Therapies for Forensic Detainees with Schizophrenia–Dementia Comorbidity. Sfera A, Andronescu L, Britt WG, Himsl K, Klein C, Rahman L, Kozlakidis Z. 2023. International Journal of Molecular Sciences. 24(21):15797. doi:10.3390/ijms242115797.
Of Soldiers and Their Ghosts: Are We Ready for a Review of PTSD Evidence? Sfera A, Anton JJ, Imran H, Kozlakidis Z, Klein C, Osorio C. 2023. BioMed. 3(4):484–506. doi:10.3390/biomed3040039.
Biomarkers in CNS-Originating Extracellular Vesicles for Parkinson’s Disease and Multiple System Atrophy. Taha, H. 2023. ProQuest. 30529443.
Tau seed amplification assay reveals relationship between seeding and pathological forms of tau in Alzheimer’s disease brain. Frey B, Holzinger D, Taylor KC, Ehrnhoefer DE, Striebinger A, Biesinger S, Gasparini L, O’Neill M, Wegner F, Barghorn S, et al. 2023. Acta Neuropathologica Communications. 11(1). doi:10.1186/s40478-023-01676-w.
IKBKB reduces huntingtin aggregation by phosphorylating serine 13 via a non-canonical IKK pathway. Cariulo C, Martufi P, Verani M, Toledo-Sherman L, Lee R, Dominguez C, Petricca L, Caricasole A. 2023. Life Science Alliance. 6(10). doi:10.26508/lsa.202302006.
Salivary Huntingtin protein is uniquely associated with clinical features of Huntington’s disease. Parkin GM, Corey-Bloom J, Snell C, Smith H, Laurenza A, Daldin M, Bresciani A, Thomas EA. 2023. Scientific Reports. 13(1):1034. doi:10.1038/s41598-023-28019-y.
Development of a Novel Electrochemiluminescence ELISA for Quantification of α-Synuclein Phosphorylated at Ser¹²⁹ in Biological Samples. Dutta S, Hornung S, Hash Brown Taha, Biggs K, Siddique I, Chamoun LM, Hedieh Shahpasand‐Kroner, Lantz C, Herrera‐Vaquero M, Stefanova N, et al. 2023. ACS Chemical Neuroscience. 14(7):1238–1248. doi:10.1021/acschemneuro.2c00676.
Nasal administration of anti-CD3 monoclonal antibody ameliorates disease in a mouse model of Alzheimer’s disease. Lopes J, Zhang X, Mayrink J, Tatematsu BK, Guo L, LeServe DS, Hadi Abou-El-Hassan, Feng R, Dalton ML, Gerhardt M, et al. 2023. Proceedings of the National Academy of Sciences of the United States of America. 120(37). doi:10.1073/pnas.2309221120.
Quantifying mutant huntingtin protein in human cerebrospinal fluid to support the development of huntingtin-lowering therapies. Vauleon S, Schutz K, Massonnet B, Gruben N, Manchester M, Buehler A, Schick E, Boak L, Hawellek DJ. 2023. Scientific Reports. 13(1). doi:10.1038/s41598-023-32630-4.
Understanding the role of the hematopoietic niche in Huntington’s disease’s phenotypic expression: in vivo evidence using a parabiosis model. Rieux M, Alpaugh M, Salem S, Siddu A, Saint-Pierre M, Denis HL, Rohweder H, Herrmann F, Bazenet C, Lacroix S, et al. 2023. Neurobiology of Disease. 180:106091–106091. doi:10.1016/j.nbd.2023.106091.
Saliva as a Relevant Biofluid for Huntington’s Disease Biomarker Research. Granger SW, Thomas EA. 2023. Contemporary Clinical Neuroscience. 77–97. doi:10.1007/978-3-031-32815-2_4.
Abundant Aβ fibrils in ultracentrifugal supernatants of aqueous extracts from Alzheimer’s disease brains. Stern AM, Yang Y, Jin S-X, Yamashita K, Meunier AL, Li W, Cai Y, Ericsson M, Liu L, Goedert M, et al. 2023. Neuron. doi:10.1016/j.neuron.2023.04.007.
Linking biomarkers with healthy lifestyle outcomes after stroke: Supplementary results of a 12-month randomized controlled trial. Swank C, McShan E, Bottiglieri T, Zurawski S, Callender L, Bennett M, Dubiel R, Driver S. 2023. Nutrition, Metabolism and Cardiovascular Diseases. doi:10.1016/j.numecd.2023.09.007.
Pharmacological PINK1 activation ameliorates Pathology in Parkinson’s Disease models. Chin RM, Rakhit R, Ditsworth D, Wang C, Bartholomeus J, Liu S, Mody A, Laishu A, Eastes A, Tai C, et al. 2023 Feb 15. doi:10.1101/2023.02.14.528378.
Rational Generation of Monoclonal Antibodies Selective for Pathogenic Forms of Alpha-Synuclein. Gibbs E, Zhao B, Roman A, Plotkin SS, Peng X, Hsueh SCC, Aina A, Wang J, Shyu C, Yip CK, et al. 2022. Biomedicines. 10(9):2168. doi:10.3390/biomedicines10092168.
Plasma TDP-43 Reflects Cortical Neurodegeneration and Correlates with Neuropsychiatric Symptoms in Huntington’s Disease. Sampedro F, Saül Martínez‐Horta, Jesús Pérez‐Pérez, Rocío Pérez‐González, Horta‐Barba A, Campolongo A, Izquierdo C, Aracil‐Bolaños I, Rivas EI, Arnau Puig-Davi, et al. 2022. Clinical Neuroradiology. 32(4):1077–1085. doi:10.1007/s00062-022-01150-5.
AAV5-miHTT-mediated huntingtin lowering improves brain health in a Huntington’s disease mouse model. Thomson SB, Stam A, Brouwers C, Fodale V, Bresciani A, Vermeulen M, Mostafavi S, Petkau TL, Hill A, Yung A, et al. 2022. Brain. 146(6):2298–2315. doi:10.1093/brain/awac458.
Brain Alterations in Aged OVT73 Sheep Model of Huntington’s Disease: An MRI Based Approach. Taghian T, Gallagher J, Batcho E, Pullan C, Kuchel T, Denney T, Perumal R, Moore S, Muirhead R, Herde P, et al. 2022. Journal of Huntington’s Disease. 11(4):391–406. doi:10.3233/JHD-220526.
Quantifying Huntingtin Protein in Human Cerebrospinal Fluid Using a Novel Polyglutamine Length-Independent Assay. Fodale V, Pintauro R, Daldin M, Spiezia MC, Macdonald D, Bresciani A. 2022. Journal of Huntington’s Disease. 11(3):291–305. doi:10.3233/jhd-220527.
ULTRASENSITIVE NEUROFILAMENT LIGHT CHAIN (NfL) IMMUNOASSAY FOR THE SMCxPRO™ ASSAY PLATFORM. Wiese R, Alex C.‐T. Ko, Sweetwater R, Fiske J. 2022. Alzheimer’s & Dementia. 18(S6). doi:10.1002/alz.066064.
Increased levels of the synaptic proteins PSD-95, SNAP-25, and neurogranin in the cerebrospinal fluid of patients with Alzheimer’s disease. Kivisäkk P, Carlyle BC, Sweeney T, Quinn JP, Ramirez CE, Trombetta BA, Mendes M, Brock M, Rubel C, Czerkowicz J, et al. 2022. Alzheimer’s Research & Therapy. 14(1). doi:10.1186/s13195-022-01002-x.
Identification of mild cognitive impairment subtypes predicting conversion to Alzheimer’s disease using multimodal data. Kikuchi M, Kobayashi K, Itoh S, Kasuga K, Miyashita A, Ikeuchi T, Yumoto E, Kosaka Y, Fushimi Y, Takeda T, et al. 2022 Aug. Computational and Structural Biotechnology Journal. doi:10.1016/j.csbj.2022.08.007.
Generation and application of semi-synthetic p-Tau181 calibrator for immunoassay calibration. Liu L, Cai Y, Lauro BM, Meunier AL, Chhatwal J, Selkoe DJ. 2022. Biochemical and Biophysical Research Communications. 611:85–90. doi:10.1016/j.bbrc.2022.04.077.
Reply to: “Comment on: Polyglutamine-Expanded Ataxin-3: A Target Engagement Marker for Spinocerebellar Ataxia Type 3 in Peripheral Blood.” Hübener-Schmid J, Kuhlbrodt K, Peladan J, European Spinocerebellar Ataxia Type 3/Machado-Joseph Disease Initiative (ESMI) Study Group, Rieß O. 2022. Movement Disorders: Official Journal of the Movement Disorder Society. 37(5):1121–1122. doi:10.1002/mds.29003.
A study of the longitudinal changes in multiple cerebrospinal fluid and volumetric magnetic resonance imaging biomarkers on converter and non‐converter Alzheimer’s disease subjects with consideration for their amyloid beta status. Morar U, Izquierdo W, Martin H, Forouzannezhad P, Zarafshan E, Unger E, Bursac Z, Cabrerizo M, Barreto A, Vaillancourt DE, et al. 2022. Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring. 14(1). doi:10.1002/dad2.12258.
Brain Biomarkers: Follow-Up of RNA Expression Discovery Approach: CSF Assays for Neurogranin, SNAP-25, and VILIP-1. Herries EM, Brada N, Sutphen CL, Fagan AM, Ladenson JH. 2021. Neuromethods.:181–221. doi:10.1007/978-1-0716-1319-1_12.
The prognostic utility of CSF neurogranin in predicting future cognitive decline in the Alzheimer’s disease continuum: A systematic review and meta-analysis with narrative synthesis. Yoong SQ, Lu J, Xing H, Gyanwali B, Tan YQ, Wu XV. 2021. Ageing Research Reviews. 72:101491. doi:10.1016/j.arr.2021.101491.
Biomarkers in Huntington’s. Killoran A. 2021 Oct 16. Neurodegenerative Diseases Biomarkers.:235–262. doi:10.1007/978-1-0716-1712-0_10.
An ultra‐sensitive immunoassay detects and quantifies soluble Aβ oligomers in human plasma. Liu L, Kwak H, Lawton TL, Jin S, Meunier AL, Dang Y, Ostaszewski B, Pietras AC, Stern AM, Selkoe DJ. 2021 Sep 22. Alzheimer’s & Dementia. doi:10.1002/alz.12457.
BIOM-15. SERUM AMYLOID-β42 AS A NONINVASIVE BIOMARKER FOR PROGNOSIS AND HISTOLOGIC FEATURES OF GLIOMA. Hwang K, Noh M, Park J, Ji SY, Han JH, Ahn KS, Kim C-Y. 2021. Neuro-Oncology. 23(Supplement_6):vi13–vi13. doi:10.1093/neuonc/noab196.046.
Recent Technological Developments in the Diagnosis and Treatment of Cerebral Edema. Deshmukh KP, Rahmani Dabbagh S, Jiang N, Tasoglu S, Yetisen AK. 2021. Advanced NanoBiomed Research. 1(11):2100001. doi:10.1002/anbr.202100001.
Widespread and sustained target engagement in Huntington’s disease minipigs upon intrastriatal microRNA-based gene therapy. Vallès A, Evers MM, Stam A, Sogorb-Gonzalez M, Brouwers C, Vendrell-Tornero C, Acar-Broekmans S, Paerels L, Klima J, Bohuslavova B, et al. 2021. Science Translational Medicine. 13(588). doi:10.1126/scitranslmed.abb8920.
Quantifying misfolded protein oligomers as drug targets and biomarkers in Alzheimer and Parkinson diseases. Kulenkampff K, Wolf Perez Adriana-M, Sormanni P, Habchi J, Vendruscolo M. 2021. Nature Reviews Chemistry. 5(4):277–294. doi:10.1038/s41570-021-00254-9.
Cognitively normal APOE ε4 carriers have specific elevation of CSF SNAP-25. Butt OH, Long JM, Henson RL, Herries E, Sutphen CL, Fagan AM, Cruchaga C, Ladenson JH, Holtzman DM, Morris JC, et al. 2021. Neurobiology of Aging. 102:64–72. doi:10.1016/j.neurobiolaging.2021.02.008.
Exogenous IGF-1 improves tau pathology and neuronal pyroptosis in high-fat diet mice with cognitive impairment. Sui G, Wang L, Yang C, Guo M, Xiong X, Chen Z, Wang F. 2021 Feb 11. doi:10.21203/rs.3.rs-158607/v1.
Intrastriatal Administration of AAV5-miHTT in Non-Human Primates and Rats Is Well Tolerated and Results in miHTT Transgene Expression in Key Areas of Huntington Disease Pathology. Spronck EA, Vallès A, Lampen MH, Montenegro-Miranda PS, Keskin S, Heijink L, Evers MM, Petry H, Deventer SJ van, Konstantinova P, et al. 2021. Brain Sciences. 11(2):129. doi:10.3390/brainsci11020129.
Identification of Mild Cognitive Impairment Subtypes Predicting Conversion to Alzheimer’s Disease Using a Heterogeneous Mixture Learning. Kikuchi M, Kobayashi K, Itoh S, Kasuga K, Miyashita A, Ikeuchi T, Yumoto E, Fushimi Y, Takeda T, Manabe S, et al. 2020 Dec 17. doi:10.21203/rs.3.rs-129455/v1.
TBK1 phosphorylates mutant Huntingtin and suppresses its aggregation and toxicity in Huntington’s disease models. Hegde RN, Chiki A, Petricca L, Martufi P, Arbez N, Mouchiroud L, Auwerx J, Landles C, Bates GP, Singh‐Bains MK, et al. 2020. The EMBO Journal. 39(17). doi:10.15252/embj.2020104671.
Identification of distinct conformations associated with monomers and fibril assemblies of mutant huntingtin. Ko J, Isas JM, Sabbaugh A, Yoo JH, Pandey NK, Chongtham A, Ladinsky M, Wu W-L, Rohweder H, Weiss A, et al. 2018. Human Molecular Genetics. 27(13):2330–2343. doi:10.1093/hmg/ddy141.
0453 INFILTRATION OF VRC01 INTO THE CEREBROSPINAL FLUID IN HUMANS IN THE RV397 STUDY. Conference on Retroviruses and Opportunistic Infections Boston USA March 8-11, 2020. Madhuu Prabhakaran, Sandeep Narpala, Lucio Gama, Donn J. Colby, Phillip Chan, Carlo Sacdalan, Khunthalee Benjapornpong, Jintanat Ananworanich, Nittaya Phanupak, Suteeraporn Pinyakorn, Trevor A. Crowell, Serena Spudich, Adrian B McDermott on behalf of the RV397 study team
Advances in amyloid beta oligomer detection applications in Alzheimer’s disease. Jamerlan A, An SSA, Hulme J. 2020. TrAC Trends in Analytical Chemistry. 129:115919. doi:10.1016/j.trac.2020.115919.
Ultrasensitive quantitative measurement of huntingtin phosphorylation at residue S13. Cariulo C, Verani M, Martufi P, Ingenito R, Finotto M, Deguire SM, Lavery DJ, Toledo-Sherman L, Lee R, Doherty EM, et al. 2020. Biochemical and Biophysical Research Communications. 521(3):549–554. doi:10.1016/j.bbrc.2019.09.097.
Target engagement in an alzheimer trial: Crenezumab lowers amyloid β oligomers in cerebrospinal fluid. Yang T, Dang Y, Ostaszewski B, Mengel D, Steffen V, Rabe C, Bittner T, Walsh DM, Selkoe DJ. 2019. Annals of Neurology. 86(2):215–224. doi:10.1002/ana.25513.
Emerging cerebrospinal fluid biomarkers in autosomal dominant Alzheimer’s disease. Schindler SE, Li Y, Todd KW, Herries EM, Henson RL, Gray JD, Wang G, Graham DL, Shaw LM, Trojanowski JQ, et al. 2019. Alzheimer’s & Dementia. 15(5):655–665. doi:10.1016/j.jalz.2018.12.019.
D06 Quantitative assays to monitor huntingtin changes in pre-clinical and clinical hd samples. Kuhlbrodt K, Baldo B, Reindl W, Carty N, Tillack K, Berson N, Mack V, Bazenet C, Herrmann F, vanderKam E, et al. 2018 Sep. Wet biomarkers. doi:10.1136/jnnp-2018-ehdn.88.
Decoding the synaptic dysfunction of bioactive human AD brain soluble Aβ to inspire novel therapeutic avenues for Alzheimer’s disease. Li S, Jin M, Liu L, Dang Y, Ostaszewski BL, Selkoe DJ. 2018. Acta Neuropathologica Communications. 6(1). doi:10.1186/s40478-018-0626-x.
APOE ε4 is associated with higher levels of CSF SNAP-25 in prodromal Alzheimer’s disease. Wang S, Zhang J, Pan T. 2018. Neuroscience Letters. 685:109–113. doi:10.1016/j.neulet.2018.08.029.
AAV5-miHTT Gene Therapy Demonstrates Broad Distribution and Strong Human Mutant Huntingtin Lowering in a Huntington’s Disease Minipig Model. Evers MM, Miniarikova J, Juhas S, Vallès A, Bohuslavova B, Juhasova J, Skalnikova HK, Vodicka P, Valekova I, Brouwers C, et al. 2018. Molecular Therapy. 26(9):2163–2177. doi:10.1016/j.ymthe.2018.06.021.
Neurogranin as Cerebrospinal Fluid Biomarker for Alzheimer Disease: An Assay Comparison Study. Willemse EAJ, De Vos A, Herries EM, Andreasson U, Engelborghs S, van der Flier WM, Scheltens P, Crimmins D, Ladenson JH, Vanmechelen E, et al. 2018. Clinical Chemistry. 64(6):927–937. doi:10.1373/clinchem.2017.283028.
Longitudinal decreases in multiple cerebrospinal fluid biomarkers of neuronal injury in symptomatic late onset Alzheimer’s disease. Sutphen CL, McCue L, Herries EM, Xiong C, Ladenson JH, Holtzman DM, Fagan AM. 2018. Alzheimer’s & Dementia. 14(7):869–879. doi:10.1016/j.jalz.2018.01.012.
A highly sensitive novel immunoassay specifically detects low levels of soluble Aβ oligomers in human cerebrospinal fluid. Yang T, O’Malley TT, Kanmert D, Jerecic J, Zieske LR, Zetterberg H, Hyman BT, Walsh DM, Selkoe DJ. 2015. Alzheimer’s Research & Therapy. 7(1):14. doi:10.1186/s13195-015-0100-y.
Interleukin 17F Level and Interferon Beta Response in Patients With Multiple Sclerosis. Hartung H-P, Steinman L, Goodin DS, Comi G, Cook S, Filippi M, O’Connor P, Jeffery DR, Kappos L, Axtell R, et al. 2013. JAMA Neurology. 70(8):1017. doi:10.1001/jamaneurol.2013.192.

Immunology/Inflammation/Virology

Targeting IL‐13 with tralokinumab normalizes type 2 inflammation in atopic dermatitis both early and at 2 years. Guttman‐Yassky E, Kenji Kabashima, Staumont‐Salle D, Nahm WK, Pauser S, Joel Correa Da Rosa, Britta Cathrina Martel, Daniel Elenius Madsen, Mads Røpke, Arlert P, et al. 2024. Allergy. 79(6):1560–1572. doi:10.1111/all.16108.
Guselkumab reduces disease- and mechanism-related biomarkers more than adalimumab in patients with psoriasis: a VOYAGE 1 substudy. Blauvelt A, Langley RG, Branigan PJ, Liu X, Chen Y, DePrimo S, Ma K, Scott B, Campbell K, Muñoz-Elías EJ, et al. 2024. JID Innovations. 4(5):100287–100287. doi:10.1016/j.xjidi.2024.100287.
Shifting the paradigm in RNA virus detection: integrating nucleic acid testing and immunoassays through single-molecule digital ELISA. Wang Z, Wei P. 2024. Frontiers in Immunology. 14. doi:10.3389/fimmu.2023.1331981.
Cedirogant in adults with psoriasis: a phase 2, randomized, placebo-controlled clinical trial. Tyring S, Moore A, Morita A, H Chih-ho Hong, Song I-H, Eccleston J, Levy G, Mohamed M-EF, Qian Y, Wu T, et al. 2024 May 3. Clinical and Experimental Dermatology. doi:10.1093/ced/llae152.
304 Dysregulated serum cytokines in association with clinical manifestations in patients with systemic lupus erythematosus. Loqmane Seridi, Leonardo S, Scott B, Gordon RM, Shu C, Fei K, Kim Hung Lo, Stevens A, Gao S. 2024 May 1. Biomarkers. doi:10.1136/lupus-2023-lupus21century.17.
Modulation of IL‐23 Signaling With Guselkumab in Biologic‐Naïve Patients Versus TNF Inhibitor‐Inadequate Responders With Active Psoriatic Arthritis. Siebert S, Coates LC, Georg Schett, Raychaudhuri SP, Chen W, Gao S, Loqmane Seridi, Chakravarty SD, Shawi M, Lavie F, et al. 2024 Jan 22. Arthritis & Rheumatology. doi:10.1002/art.42803.
Relationships between Abdominal Adipose Tissue and Neuroinflammation Using Diffusion Basis Spectrum Imaging in Midlife Obesity. Mahsa Dolatshahi, Commean P, Rahmani F, Xu Y, Liu J, Sara Hosseinzadeh Kassani, Mahshid Naghashzadeh, Lloyd L, Nguyen C, McBee-Kemper A, et al. 2024 Jan 1. doi:10.2139/ssrn.4810671.
Guselkumab More Effectively Neutralizes Psoriasis-Associated Histologic, Transcriptomic, and Clinical Measures than Ustekinumab. Campbell K, Li K, Yang F, Branigan P, M Merle Elloso, Benson J, Orlovsky Y, Chen Y, Garcet S, Krueger JG. 2023. ImmunoHorizons. 7(4):273–285. doi:10.4049/immunohorizons.2300003.
Genetic and Molecular Distinctions Between Axial Psoriatic Arthritis and Radiographic Axial Spondyloarthritis: Post Hoc Analyses from Four Phase 3 Clinical Trials. Kavanaugh A, Xenofon Baraliakos, Gao S, Chen W, Sweet K, Chakravarty SD, Song Q, Shawi M, Rahman P. 2023. Advances in Therapy. 40(5):2439–2456. doi:10.1007/s12325-023-02475-4.
Effect of guselkumab on serum biomarkers in patients with active psoriatic arthritis and inadequate response to tumor necrosis factor inhibitors: results from the COSMOS phase 3b study. Georg Schett, Chen W, Gao S, Chakravarty SD, M. Shawi, Lavie F, Zimmermann M, Sharaf MA, Coates LC, Siebert S. 2023. Arthritis Research & Therapy. 25(1). doi:10.1186/s13075-023-03125-4.
Comparison of the Inflammatory Circuits in Psoriasis Vulgaris, Non‒Pustular Palmoplantar Psoriasis, and Palmoplantar Pustular Psoriasis. Wang CQ, Haxhinasto S, Garcet S, Kunjravia N, Cueto I, Gonzalez J, Rambhia D, Harari O, Sleeman MA, Hamilton JD, et al. 2023. Journal of Investigative Dermatology. 143(1):87-97.e14. doi:10.1016/j.jid.2022.05.1094.
Quantifying inflammation using interleukin‐6 for improved phenotyping and risk stratification in acute heart failure. Michou E, Wussler D, Belkin M, Simmen C, Strebel I, Nowak A, Nikola Kozhuharov, Shrestha S, López-Ayala P, Zaid Sabti, et al. 2023. European Journal of Heart Failure. 25(2):174–184. doi:10.1002/ejhf.2767.
Neutralization of IL-13 by tralokinumab improves atopic dermatitis endotypes characterized by S. aureus colonization. Simpson E, Boguniewicz M, Eichenfield L, Røpke M, Arlert P, Schneider S, Bieber T. 2023. Journal of the American Academy of Dermatology. 89(3, Supplement):AB198. doi:10.1016/j.jaad.2023.07.792.
The Development and Characterization of a Highly Sensitive Mature TGFβ3 Assay to Evaluate Anti-TGFβ3 Target Engagement. Setiadi AF, Sperinde G, Cheu M, Liang W-C, Lin W, Mahood C, Fischer SK. 2023 Jan 26. The AAPS Journal. 25(1). doi:10.1208/s12248-023-00785-7.
Relationships of circulating CD4⁺ T cell subsets and cytokines with the risk of relapse in patients with Crohn’s disease. Duclaux-Loras R, Boschetti G, Flourie B, Roblin X, Leluduec J-B, Paul S, Almeras T, Ruel K, Buisson A, Bienvenu J, et al. 2022. Frontiers in Immunology. 13. doi:10.3389/fimmu.2022.864353.
Tralokinumab treatment improves the skin microbiota by increasing the microbial diversity in adults with moderate-to-severe atopic dermatitis: Analysis of microbial diversity in ECZTRA 1, a randomized controlled trial. Beck LA, Bieber T, Weidinger S, Tauber M, Saeki H, Irvine AD, Eichenfield LF, Werfel T, Arlert P, Jiang L, et al. 2022 Dec. Journal of the American Academy of Dermatology. doi:10.1016/j.jaad.2022.11.047.
Effect of guselkumab on serum biomarkers in Japanese palmoplantar pustulosis patients in a randomized phase 3 study. Morita A, Chen Y, Leung MWL, Kawashima N, Terui T. 2022 Dec 7. JEADV Clinical Practice. doi:10.1002/jvc2.73.
Determination of Anti-drug Antibody Affinity in Clinical Study Samples Provides a Tool for Evaluation of Immune Response Maturation. Joyce A, Shea C, You Z, Gorovits B, Lepsy C. 2022 Nov 2. The AAPS Journal. 24(6). doi:10.1208/s12248-022-00759-1.
Phase 3, multicentre, randomised, placebo-controlled study evaluating the efficacy and safety of ustekinumab in patients with systemic lupus erythematosus. Vollenhoven RF van, Kalunian KC, Dörner T, Hahn BH, Tanaka Y, Gordon RM, Shu C, Fei K, Gao S, Seridi L, et al. 2022. Annals of the Rheumatic Diseases. doi:10.1136/ard-2022-222858.
Biological impact of iberdomide in patients with active systemic lupus erythematosus. Lipsky PE, Vollenhoven R van, Dörner T, Werth VP, Merrill JT, Furie R, Petronijevic M, Velasco Zamora B, Majdan M, Irazoque-Palazuelos F, et al. 2022. Annals of the Rheumatic Diseases. doi:10.1136/annrheumdis-2022-222212.
Safety and tolerability of AAV8 delivery of a broadly neutralizing antibody in adults living with HIV: a phase 1, dose-escalation trial. Casazza JP, Cale EM, Narpala S, Yamshchikov GV, Coates EE, Hendel CS, Novik L, Holman LA, Widge AT, Apte P, et al. 2022. Nature Medicine. 28(5):1022–1030. doi:10.1038/s41591-022-01762-x.
Efficacy, Safety, and Pharmacodynamic Effects of the Bruton’s Tyrosine Kinase Inhibitor Fenebrutinib (GDC‐0853) in Systemic Lupus Erythematosus: Results of a Phase II, Randomized, Double‐Blind, Placebo‐Controlled Trial. Isenberg D, Furie R, Jones NS, Guibord P, Galanter J, Lee C, McGregor A, Toth B, Rae J, Hwang O, et al. 2021. Arthritis & Rheumatology. 73(10):1835–1846. doi:10.1002/art.41811.
Proteomic signatures of inflammatory skin diseases: a focus on atopic dermatitis. Mikhaylov D, Del Duca E, Guttman-Yassky E. 2021. Expert Review of Proteomics. 18(5):345–361. doi:10.1080/14789450.2021.1935247.
Cardiovascular biomarkers in patients with COVID-19. Mueller C, Giannitsis E, Jaffe AS, Huber K, Mair J, Cullen L, Hammarsten O, Mills NL, Möckel M, Krychtiuk K, et al. 2021. European Heart Journal Acute Cardiovascular Care. 10(3):310–319. doi:10.1093/ehjacc/zuab009.
A sensitive method to quantify HIV-1 antibodies in mucosal samples. Prabhakaran M, Narpala S, Andrews SF, O’Connell S, Lin CL, Coates EE, Flach B, Ledgerwood JE, McDermott AB. 2021. Journal of Immunological Methods. 491:112995. doi:10.1016/j.jim.2021.112995.
Pharmacodynamic analysis of apremilast in Japanese patients with moderate to severe psoriasis: Results from a phase 2b randomized trial. Imafuku S, Nemoto O, Okubo Y, Komine M, Schafer P, Petric R, Ohtsuki M. 2020. The Journal of Dermatology. 48(1):80–84. doi:10.1111/1346-8138.15596.
P273 Ustekinumab and guselkumab treatment results in differences in serum IL-17A, IL-17F and CRP levels in PsA patients: a comparison from ustekinumab Phase 3 and guselkumab Phase 2 programmes. Siebert S, Loza MJ, Song Q, Gorecki PC, McInnes IB, Sweet K. 2020. Rheumatology. 59(Supplement_2). doi:10.1093/rheumatology/keaa111.266.
Upadacitinib Treatment Induces Significant Improvements in Th2 (Eosinophil Count, Serum CCL17/18/26) and Th22 (IL-22) Levels in Atopic Dermatitis That Are Associated With Improvements in Itch and Clinical Severity. Presented at the Revolutionizing Atopic Dermatitis (RAD) 2020 Virtual Congress, April 5, 2020, Chicago, Illinois. Emma Guttman-Yassky, Ana B. Pavel, Jonathan I. Silverberg, Stephan Weidinger, Julie Parmentier, Henrique D. Teixeira, Feng Hong, Lisa A. Beck.
Early Quantification of Systemic Inflammatory Proteins Predicts Long-Term Treatment Response to Tofacitinib and Etanercept. Tomalin LE, Kim J, Correa da Rosa J, Lee J, Fitz LJ, Berstein G, Valdez H, Wolk R, Krueger JG, Suárez-Fariñas M. 2020. Journal of Investigative Dermatology. 140(5):1026–1034. doi:10.1016/j.jid.2019.09.023.
Apremilast mechanism of efficacy in systemic-naive patients with moderate plaque psoriasis: Pharmacodynamic results from the UNVEIL study. Strober B, Alikhan A, Lockshin B, Shi R, Cirulli J, Schafer P. 2019. Journal of Dermatological Science. 96(3):126–133. doi:10.1016/j.jdermsci.2019.09.003.
Guselkumab Efficacy after Withdrawal Is Associated with Suppression of Serum IL-23-Regulated IL-17 and IL-22 in Psoriasis: VOYAGE 2 Study. Gordon KB, Armstrong AW, Foley P, Song M, Shen Y-K, Li S, Muñoz-Elías EJ, Branigan P, Liu X, Reich K. 2019. Journal of Investigative Dermatology. 139(12):2437-2446.e1. doi:10.1016/j.jid.2019.05.016.
Secukinumab Treatment Does Not Alter the Pharmacokinetics of the Cytochrome P450 3A4 Substrate Midazolam in Patients With Moderate to Severe Psoriasis. Bruin G, Hasselberg A, Koroleva I, Milojevic J, Calonder C, Soon R, Woessner R, Pariser DM, Boutouyrie‐Dumont B. 2019. Clinical Pharmacology & Therapeutics. 106(6):1380–1388. doi:10.1002/cpt.1558.
Impact of high‐altitude therapy on type‐2 immune responses in asthma patients. Boonpiyathad T, Capova G, Duchna H, Croxford AL, Farine H, Dreher A, Clozel M, Schreiber J, Kubena P, Lunjani N, et al. 2019. Allergy. 75(1):84–94. doi:10.1111/all.13967.
Combining single molecule counting with bead-based multiplexing to quantify biological inflammation time course following skeletal muscle injury. Tanner EA, Gary MA, Davis AA, McFarlin BK. 2019. Methods. 158:77–80. doi:10.1016/j.ymeth.2018.11.013.
Profiling Immune Expression to Consider Repurposing Therapeutics for the Ichthyoses. Paller AS. 2019. Journal of Investigative Dermatology. 139(3):535–540. doi:10.1016/j.jid.2018.08.027.
Clinical utility of circulating interleukin-6 concentrations in the detection of functionally relevant coronary artery disease. Walter J, Tanglay Y, du Fay de Lavallaz J, Strebel I, Boeddinghaus J, Twerenbold R, Doerflinger S, Puelacher C, Nestelberger T, Wussler D, et al. 2019. International Journal of Cardiology. 275:20–25. doi:10.1016/j.ijcard.2018.10.029.
Synergistic cytokine effects as apremilast response predictors in patients with psoriasis. Garcet S, Nograles K, Correa da Rosa J, Schafer PH, Krueger JG. 2018. Journal of Allergy and Clinical Immunology. 142(3):1010-1013.e6. doi:10.1016/j.jaci.2018.05.039.
An integrated model of alopecia areata biomarkers highlights both TH1 and TH2 upregulation. Song T, Pavel AB, Wen H-C, Malik K, Estrada Y, Gonzalez J, Hashim PW, Nia JK, Baum D, Kimmel G, et al. 2018. Journal of Allergy and Clinical Immunology. 142(5):1631-1634.e13. doi:10.1016/j.jaci.2018.06.029.
Ichthyosis molecular fingerprinting shows profound TH17 skewing and a unique barrier genomic signature. Malik K, He H, Huynh TN, Tran G, Mueller K, Doytcheva K, Renert-Yuval Y, Czarnowicki T, Magidi S, Chou M, et al. 2019. Journal of Allergy and Clinical Immunology. 143(2):604–618. doi:10.1016/j.jaci.2018.03.021.
Association between serum interleukin-17A and clinical response to tofacitinib and etanercept in moderate to severe psoriasis. Fitz L, Zhang W, Soderstrom C, Fraser S, Lee J, Quazi A, Wolk R, Mebus CA, Valdez H, Berstein G. 2018. Clinical and Experimental Dermatology. 43(7):790–797. doi:10.1111/ced.13561.
The NET-effect of combining rituximab with belimumab in severe systemic lupus erythematosus. Kraaij T, Kamerling SWA, de Rooij ENM, van Daele PLA, Bredewold OW, Bakker JA, Bajema IM, Scherer HU, Toes REM, Huizinga TJW, et al. 2018. Journal of Autoimmunity. 91:45–54. doi:10.1016/j.jaut.2018.03.003.
Serum from Asian patients with atopic dermatitis is characterized by T H 2/T H 22 activation, which is highly correlated with nonlesional skin measures. Wen H-C, Czarnowicki T, Noda S, Malik K, Pavel AB, Nakajima S, Honda T, Shin JU, Lee H, Chou M, et al. 2018. Journal of Allergy and Clinical Immunology. 142(1):324-328.e11. doi:10.1016/j.jaci.2018.02.047.
Evaluation of highly sensitive immunoassay technologies for quantitative measurements of sub-pg/mL levels of cytokines in human serum. Yeung D, Ciotti S, Purushothama S, Gharakhani E, Kuesters G, Schlain B, Shen C, Donaldson D, Mikulskis A. 2016. Journal of Immunological Methods. 437:53–63. doi:10.1016/j.jim.2016.08.003.
Due diligence in the characterization of matrix effects in a total IL-13 Singulex^® method. Fraser S, Soderstrom C. 2014. Bioanalysis. 6(8):1123–1129. doi:10.4155/bio.14.42.
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Todd J, Simpson P, Estis J, Torres V, Wub AHB. 2013. Cytokine. 64(3):660–665. doi:10.1016/j.cyto.2013.09.018.
Interleukin 17F Level and Interferon Beta Response in Patients With Multiple Sclerosis. Hartung H-P, Steinman L, Goodin DS, Comi G, Cook S, Filippi M, O’Connor P, Jeffery DR, Kappos L, Axtell R, et al. 2013. JAMA Neurology. 70(8):1017. doi:10.1001/jamaneurol.2013.192.
Quantitative determination of human interleukin 22 (IL-22) in serum using Singulex^®-Erenna^® Technology. Shukla R, Santoro J, Bender FC, Laterza OF. 2013. Journal of Immunological Methods. 390(1-2):30–34. doi:10.1016/j.jim.2013.01.002.
Analytical validation of a highly sensitive microparticle-based immunoassay for the quantitation of IL-13 in human serum using the Erenna^® immunoassay system. St. Ledger K, Agee SJ, Kasaian MT, Forlow SB, Durn BL, Minyard J, Lu QA, Todd J, Vesterqvist O, Burczynski ME. 2009. Journal of Immunological Methods. 350(1-2):161–170. doi:10.1016/j.jim.2009.08.012.

Cardiovascular

WCN24-972 INVESTIGATION OF URINE MYO-INOSITOL OXYGENASE AS A BIOMARKER OF ACUTE KIDNEY INJURY IN CARDIOPULMONARY BYPASS SURGERY PATIENTS. Gaut J, Herries E, Brada N, Farnsworth C, Satoru Kudose, Damiano R, Ladenson J, Vijayan A. 2024. Kidney International Reports. 9(4):S13–S14. doi:10.1016/j.ekir.2024.02.089.
Ultrasensitive Upconversion Nanoparticle Immunoassay for Human Serum Cardiac Troponin I Detection Achieved with Resonant Waveguide Grating. Tseng Y-T, Chiu Y-C, Pham V-D, Wu W-H, Thanh Thu Le-Vu, Wang C-H, Kuo S-W, Michael W.Y. Chan, Lin C-H, Li S-C, et al. 2024. ACS Sensors. 9(1):455–463. doi:10.1021/acssensors.3c02240.
Effect of 2022 ACC/AHA/HFSA Criteria on Stages of Heart Failure in a Pooled Community Cohort. Reza Mohebi, Wang D, Lau ES, Parekh JK, Allen N, Psaty BM, Benjamin EJ, Levy D, Wang TJ, Shah SJ, et al. 2023. 81(23):2231–2242. doi:10.1016/j.jacc.2023.04.007.
Association of Circulating Cardiomyocyte Cell-Free DNA With Cancer Therapy-Related Cardiac Dysfunction in Patients Undergoing Treatment for ERBB2-Positive Breast Cancer. Yu AF, Moore ZR, Moskowitz CS, Liu JE, Dang CT, Ramanathan L, Oeffinger KC, Steingart RM, Schmitt AM. 2023. JAMA cardiology. 8(7):697–702. doi:10.1001/jamacardio.2023.1229.
Judging the clinical suitability of analytical performance of cardiac troponin assays. Clinical Chemistry and Laboratory Medicine (CCLM). Krintus M, Panteghini M. 2023 Feb 20. 0(0). doi:10.1515/cclm-2023-0027.
Cardiac Troponin I in Patients Undergoing Percutaneous and Surgical Myocardial Revascularization: Comparison of Analytical Methods. Vendramini SP do A, Strunz CMC, Hueb WA, Mansur A de P. 2023. Diagnostics. 13(7):1316. doi:10.3390/diagnostics13071316.
Anthracyclines and Biomarkers of Myocardial Injury: The Effect of Remote Ischemic Conditioning. Mallouppas M, Chung R, Ghosh AK, Macklin A, Yellon DM, Walker JM. 2023. JACC: CardioOncology. 5(3):343–355. doi:10.1016/j.jaccao.2023.03.008.
Risk factors for cardiovascular disease among individuals with hepatic steatosis. Hepatology Communications. Karády J, Ferencik M, Mayrhofer T, Meyersohn NM, Bittner DO, Staziaki PV, Szilveszter B, Hallett TR, Lu MT, Puchner SB, et al. 2022. 6(12):3406–3420. doi:https://doi.org/10.1002/hep4.2090.
CSL112 (Apolipoprotein A-I [Human]) Strongly Enhances Plasma Apoa-I and Cholesterol Efflux Capacity in Post-Acute Myocardial Infarction Patients: A PK/PD Substudy of the AEGIS-I Trial. C Michael Gibson, Muhammad Junaid Munir, Serge Korjian, Chi G, Phillips AT, Sahar Memar Montazerin, Duffy D, Zheng B, Heise M, Liss C, et al. 2022. Journal of Cardiovascular Pharmacology and Therapeutics. 27:107424842211215-107424842211215. doi:10.1177/10742484221121507.
Abdominal Obesity May Confound the Accuracy of Cardiovascular Risk Prediction in Rheumatoid Arthritis; Can Coronary Atherosclerosis Imaging and Biomarkers Help? Karpouzas G, Ormseth S, Hernandez E, Budoff M. 2022. Annals of the Rheumatic Diseases. 81(Suppl 1):88–88. doi:10.1136/annrheumdis-2022-eular.1885.
Inflammatory activation biomarker profile after marathon running and its impact on cardiovascular stress in amateur middle-aged male runners. Kosowski M, Młynarska K, Chmura J, Kustrzycka-Kratochwil D, Todd J, Jankowska E, Reczuch K, Ponikowski P. 2022 Nov 18. Advances in Clinical and Experimental Medicine. 32(4). doi:10.17219/acem/155018.
Serial measurements of protein and microRNA biomarkers to specify myocardial infarction subtypes. Schulte C, Singh B, Theofilatos K, Sörensen NA, Lehmacher J, Hartikainen T, Haller PM, Westermann D, Zeller T, Blankenberg S, et al. 2022 Sept. Journal of Molecular and Cellular Cardiology Plus. 1:100014. doi:10.1016/j.jmccpl.2022.100014.
Comparison of Point-of-Care and Highly Sensitive Laboratory Troponin Testing in Patients Suspicious of Acute Myocardial Infarction and Its Efficacy in Clinical Outcome. Mohammadzadeh S, Matani N, Soleimani N, Bazrafshan drissi H. 2022. Severino P, editor. Cardiology Research and Practice. 2022:1–7. doi:10.1155/2022/6914979.
A 0/1h-algorithm using cardiac myosin-binding protein C for early diagnosis of myocardial infarction. Kaier TE, Twerenbold R, Lopez-Ayala P, Nestelberger T, Boeddinghaus J, Alaour B, Huber I-M, Zhi Y, Koechlin L, Wussler D, et al. 2022 Feb 12. European Heart Journal Acute Cardiovascular Care. doi:10.1093/ehjacc/zuac007.
Biological variation of cardiac myosin-binding protein C in healthy individuals. Alaour B, Omland T, Torsvik J, Kaier TE, Sylte MS, Strand H, Quraishi J, McGrath S, Williams L, Meex S, et al. 2021. Clinical Chemistry and Laboratory Medicine (CCLM). 60(4):576–583. doi:10.1515/cclm-2021-0306.
Cardiovascular biomarkers in patients with COVID-19. Mueller C, Giannitsis E, Jaffe AS, Huber K, Mair J, Cullen L, Hammarsten O, Mills NL, Möckel M, Krychtiuk K, et al. 2021. European Heart Journal Acute Cardiovascular Care. 10(3):310–319. doi:10.1093/ehjacc/zuab009.
Interleukin-6 and Outcomes in Acute Heart Failure: An ASCEND-HF Substudy. Perez AL, GRODIN JL, CHAIKIJURAJAI T, WU Y, HERNANDEZ AF, BUTLER J, METRA M, FELKER GM, VOORS AA, MCMURRAY JJ, et al. 2021.Journal of Cardiac Failure. 27(6):670–676. doi:10.1016/j.cardfail.2021.01.006.
Clinical relevance of biological variation of cardiac troponins. Clerico A, Padoan A, Zaninotto M, Passino C, Plebani M. 2020. Clinical Chemistry and Laboratory Medicine (CCLM). 59(4):641–652. doi:10.1515/cclm-2020-1433.
Biological Variation of Cardiac Troponins in Health and Disease: A Systematic Review and Meta-analysis. Diaz-Garzon J, Fernandez-Calle P, Sandberg S, Özcürümez M, Bartlett WA, Coskun A, Carobene A, Perich C, Simon M, Marques F, et al. 2021. Clinical Chemistry. 67(1):256–264. doi:10.1093/clinchem/hvaa261.
Early Rule-Out Strategies in the Emergency Department Utilizing High-Sensitivity Cardiac Troponin Assays. Lopez-Ayala P, Boeddinghaus J, Koechlin L, Nestelberger T, Mueller C. 2020. Clinical Chemistry. 67(1):114–123. doi:10.1093/clinchem/hvaa226.
Differential associations of cardiac troponin T and cardiac troponin I with coronary artery pathology and dynamics in response to short-duration exercise. Tveit SH, Cwikiel J, Myhre PL, Omland T, Berge E, Seljeflot I, Flaa A. 2021. Clinical Biochemistry. 88:23–29. doi:10.1016/j.clinbiochem.2020.11.005.
Advances in point-of-care testing for cardiovascular diseases. Regan B, O’Kennedy R, Collins D. 2021. Advances in Clinical Chemistry.:1–70. doi:10.1016/bs.acc.2020.09.001.
Cardiac Myosin‐Binding Protein C to Diagnose Acute Myocardial Infarction in the Pre‐Hospital Setting. Kaier TE, Stengaard C, Marjot J, Sørensen JT, Alaour B, Stavropoulou‐Tatla S, Terkelsen CJ, Williams L, Thygesen K, Weber E, et al. 2019. Journal of the American Heart Association. 8(15). doi:10.1161/jaha.119.013152.
Clinical determinants of plasma cardiac biomarkers in patients with stable chest pain. Bing R, Henderson J, Hunter A, Williams MC, Moss AJ, Shah ASV, McAllister DA, Dweck MR, Newby DE, Mills NL, et al. 2019. Heart. 105(22):1748–1754. doi:10.1136/heartjnl-2019-314892.
Droplet digital PCR of serum miR-499, miR-21 and miR-208a for the detection of functionally relevant coronary artery disease. Hortmann M, Walter JE, Benning L, Follo M, Mayr RM, Honegger U, Robinson S, Stallmann D, Duerschmied D, Twerenbold R, et al. 2019. International Journal of Cardiology. 275:129–135. doi:10.1016/j.ijcard.2018.08.031.
High-sensitivity cardiac troponin T increases after stress echocardiography. Samaha E, Brown J, Brown F, Martinez SC, Scott M, Jaffe AS, Davila-Roman VG, Nagele P. 2019. Clinical Biochemistry. 63:18–23. doi:10.1016/j.clinbiochem.2018.11.013.
Clinical utility of circulating interleukin-6 concentrations in the detection of functionally relevant coronary artery disease. Walter J, Tanglay Y, du Fay de Lavallaz J, Strebel I, Boeddinghaus J, Twerenbold R, Doerflinger S, Puelacher C, Nestelberger T, Wussler D, et al. 2019. International Journal of Cardiology. 275:20–25. doi:10.1016/j.ijcard.2018.10.029.
DT‐02‐03: TARGET ENGAGEMENT IN AN AD TRIAL: CRENEZUMAB LOWERS Aβ OLIGOMER LEVELS IN CSF. Yang T, Dang Y, Ostaszewski B, Mengel D, Steffen V, Rabe C, Bittner T, Walsh DM, Selkoe DJ. 2006. Alzheimer’s & Dementia. 14(7S_Part_31). doi:10.1016/j.jalz.2018.07.012.
1086Derivation and validation of a 0/1h-algorithm to diagnose myocardial infarction using cardiac myosin-binding protein c - direct comparison to hs-cTnI. Kaier TE, Twerenbold R, Alaour B, Badertscher P, Puelacher C, Marjot J, Boeddinghaus J, Nestelberger T, Wildi K, Wussler D, et al. 2018. European Heart Journal. 39(suppl_1). doi:10.1093/eurheartj/ehy565.1086.
Screening for Cardiac Disease with Genetic Risk Scoring, Advanced ECG, Echocardiography, Protein Biomarkers and Metabolomics. Gladding P, Dugo C, Wynne Y, Semple H, Smith K, Shepherd P, Zarate E, Larsen P, Vilas-Boas S. 2018. Heart, Lung and Circulation. 27:S8. doi:10.1016/j.hlc.2018.05.117.
High-Sensitivity Cardiac Troponin I and the Diagnosis of Coronary Artery Disease in Patients With Suspected Angina Pectoris. Adamson PD, Hunter A, Madsen DM, Shah ASV, McAllister DA, Pawade TA, Williams MC, Berry C, Boon NA, Flather M, et al. 2018. Circulation: Cardiovascular Quality and Outcomes. 11(2). doi:10.1161/circoutcomes.117.004227.
Single‐Molecule Counting of High‐Sensitivity Troponin I in Patients Referred for Diagnostic Angiography: Results From the CASABLANCA (Catheter Sampled Blood Archive in Cardiovascular Diseases) Study. McCarthy CP, Ibrahim NE, Lyass A, Li Y, Gaggin HK, Simon ML, Mukai R, Gandhi P, Kelly N, Motiwala SR, et al. 2018. Journal of the American Heart Association. 7(6). doi:10.1161/jaha.117.007975.
Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect. Zhou W, Li K, Wei Y, Hao P, Chi M, Liu Y, Wu Y. 2018. Biosensors and Bioelectronics. 106:99–104. doi:10.1016/j.bios.2018.01.061.
CARDIAC TROPONIN I AND SUBCLINICAL CARDIOVASCULAR DISEASE. Joo E, Darabian S, Nozari Y, Vahoumeni R, Sheidaee N, Wade NB, Budoff M. 2018. Journal of the American College of Cardiology. 71(11):A130. doi:10.1016/s0735-1097(18)30671-5.
Prospective Validation of a Biomarker-Based Rule Out Strategy for Functionally Relevant Coronary Artery Disease. Walter JE, Honegger U, Puelacher C, Mueller D, Wagener M, Schaerli N, Strebel I, Twerenbold R, Boeddinghaus J, Nestelberger T, et al. 2018. Clinical Chemistry. 64(2):386–395. doi:10.1373/clinchem.2017.277210.
Comprehensive Age and Sex 99th Percentiles for a High-Sensitivity Cardiac Troponin I Assay. Estis J, Wu AHB, Todd J, Bishop J, Sandlund J, Kavsak PA. 2018. Clinical Chemistry. 64(2):398–399. doi:10.1373/clinchem.2017.276972.
Prevalence, predictors and clinical outcome of residual congestion in acute decompensated heart failure. Rubio-Gracia J, Demissei BG, ter Maaten JM, Cleland JG, O’Connor CM, Metra M, Ponikowski P, Teerlink JR, Cotter G, Davison BA, et al. 2018. International Journal of Cardiology. 258:185–191. doi:10.1016/j.ijcard.2018.01.067.
Quantifying the Release of Biomarkers of Myocardial Necrosis from Cardiac Myocytes and Intact Myocardium. Marjot J, Kaier TE, Martin ED, Reji SS, Copeland O, Iqbal M, Goodson B, Hamren S, Harding SE, Marber MS. 2017. Clinical Chemistry. 63(5):990–996. doi:10.1373/clinchem.2016.264648.
Can a Point-of-Care Troponin I Assay be as Good as a Central Laboratory Assay? A MIDAS Investigation. Peacock WF, Diercks D, Birkhahn R, Singer AJ, Hollander JE, Nowak R, Safdar B, Miller CD, Peberdy M, Counselman F, et al. 2016. Annals of Laboratory Medicine. 36(5):405–412. doi:10.3343/alm.2016.36.5.405.
The development and application of a high-sensitivity immunoassay for cardiac myosin–binding protein C. Marjot J, Liebetrau C, Goodson RJ, Kaier T, Weber E, Heseltine P, Marber MS. 2016. Translational Research. 170:17-25.e5. doi:10.1016/j.trsl.2015.11.008.
Longitudinal studies of cardiac troponin I concentrations in serum from male cynomolgus monkeys: resting values and effects of oral and intravenous dosing on biologic variability. Schultze AE, Anderson JM, Kern TG, Justus RW, Lee H-YC, Zieske LR, Goodson RJ, Florey SH. 2015. Veterinary Clinical Pathology. 44(3):465–471. doi:10.1111/vcp.12272.
Prognostic performance of a high-sensitivity assay for cardiac troponin I after non-ST elevation acute coronary syndrome: Analysis from MERLIN-TIMI 36. Bonaca MP, O’Malley RG, Murphy SA, Jarolim P, Conrad MJ, Braunwald E, Sabatine MS, Morrow DA. 2014. European Heart Journal: Acute Cardiovascular Care. 4(5):431–440. doi:10.1177/2048872614564081.
The utility of serum biomarkers to detect myocardial alterations induced by Imatinib in rats. Herman E, Knapton A, Zhang J, Estis J, Todd J, Lipshultz S. 2014. Pharmacology Research & Perspectives. 2(1). doi:10.1002/prp2.15.
Baseline Serum Cardiac Troponin I Concentrations in Sprague-Dawley, Spontaneous Hypertensive, Wistar, Wistar-Kyoto, and Fisher Rats as Determined with an Ultrasensitive Immunoassay. Herman E, Knapton A, Rosen E, Zhang J, Estis J, Agee SJ, Lu Q-A, Todd JA, Lipshultz SE. 2011. Toxicologic Pathology. 39(4):653–663. doi:10.1177/0192623311406931.
Defining the serum 99th percentile in a normal reference population measured by a high-sensitivity cardiac troponin I assay. Apple FS, Simpson PA, Murakami MM. 2010. Clinical Biochemistry. 43(12):1034–1036. doi:10.1016/j.clinbiochem.2010.05.014.
Assessment of the Toxicity of Hydralazine in the Rat Using an Ultrasensitive Flow-based Cardiac Troponin I Immunoassay.Mikaelian I, Coluccio D, Hirkaler GM, Downing JC, Rasmussen E, Todd J, Estis J, Lu QA, Nicklaus R. 2009. Toxicologic Pathology. 37(7):878–881. doi:10.1177/0192623309351894.
Ultrasensitive Cross-species Measurement of Cardiac Troponin-I Using the Erenna® Immunoassay System. Schultze AE, Konrad RJ, Credille KM, Lu QA, Todd J. 2008. Toxicologic Pathology. 36(6):777–782. doi:10.1177/0192623308322016.

PK/PD AND IMMUNOGENICITY

Impact of LS Mutation on Pharmacokinetics of Preventive HIV Broadly Neutralizing Monoclonal Antibodies: A Cross-Protocol Analysis of 16 Clinical Trials in People without HIV. Mayer BT, Zhang L, deCamp AC, Yu C, Sato A, Angier H, Seaton KE, Yates N, Ledgerwood JE, Mayer K, et al. 2024. Pharmaceutics. 16(5):594–594. doi:https://doi.org/10.3390/pharmaceutics16050594.
Safety, tolerability, and pharmacokinetics of single‐ and multiple‐ascending doses of olamkicept: Results from randomized, placebo‐controlled, first‐in‐human phase I trials. Wagner F, Schreiber S, Bagger Y, Bruzelius K, Falahati A, Sternebring O, Ravi A, Philippe Pinton. 2024. Clinical and Translational Science. 17(5). doi:10.1111/cts.13832.
Utilizing PK and PD Biomarkers to Guide the First-in-Human Starting Dose Selection of MTBT1466A: A Novel Humanized Monoclonal Anti-TGFβ3 Antibody for the Treatment of Fibrotic Diseases. Yadav R, Sukumaran S, Lutman J, Mitra MS, Halpern W, Sun T, Setiadi AF, Neighbors M, Sheng XR, Yip V, et al. 2023. Journal of Pharmaceutical Sciences. 112(11):2910–2920. doi:10.1016/j.xphs.2023.07.005.
White Paper on Recent Issues in Bioanalysis: Enzyme Assay Validation, BAV for Primary End Points, Vaccine Functional Assays, Cytometry in Tissue, LBA in Rare Matrices, Complex NAb Assays, Spectral Cytometry, Endogenous Analytes, Extracellular Vesicles Part 2 – Recommendations on Biomarkers/CDx, Flow Cytometry, Ligand-Binding Assays Development & Validation; Emerging Technologies; Critical Reagents Deep Characterization. Sumner G, Keller S, Huleatt JW, Staack RF, Wagner L, Mitra Azadeh, Abbas Bandukwala, Cao L, Du X, Salinas G, et al. 2023. Bioanalysis. 15(15):861–903. doi:10.4155/bio-2023-0151.
A Phase 1 Study of the DNA-PK Inhibitor Peposertib in Combination With Radiation Therapy With or Without Cisplatin in Patients With Advanced Head and Neck Tumors. Samuels M, Falkenius J, Bar-Ad V, Dunst J, van Triest B, Yachnin J, Rodriguez-Gutierrez A, Kuipers M, You X, Sarholz B, et al. 2023. International Journal of Radiation Oncology*Biology*Physics. doi:10.1016/j.ijrobp.2023.09.024.
White Paper on Recent Issues in Bioanalysis: FDA Draft Guidance on Immunogenicity Information in Prescription Drug Labeling, LNP & Viral Vectors Therapeutics/Vaccines Immunogenicity, Prolongation Effect, ADA Affinity, Risk-based Approaches, NGS, qPCR, ddPCR Assays (Part 3 - Recommendations on Gene Therapy, Cell Therapy, Vaccines Immunogenicity & Technologies; Immunogenicity & Risk Assessment of Biotherapeutics and Novel Modalities; NAb Assays Integrated Approach). Pan L, Mora J, Walravens K, Wagner L, Hopper S, Loo L, Bettoun D, Bond S, Dessy F, Downing S, et al. 2023. Bioanalysis. 15(14):773–814. doi:10.4155/bio-2023-0135.
Determination of Anti-drug Antibody Affinity in Clinical Study Samples Provides a Tool for Evaluation of Immune Response Maturation. Joyce A, Shea C, You Z, Gorovits B, Lepsy C. 2022. The AAPS Journal. 24(6). doi:10.1208/s12248-022-00759-1.
Safety and tolerability of AAV8 delivery of a broadly neutralizing antibody in adults living with HIV: a phase 1, dose-escalation trial. Casazza JP, Cale EM, Narpala S, Yamshchikov GV, Coates EE, Hendel CS, Novik L, Holman LA, Widge AT, Apte P, et al. 2022. Nature Medicine. 28(5):1022–1030. doi:10.1038/s41591-022-01762-x.

General Applications

2023 White Paper on Recent Issues in Bioanalysis: EU IVDR 2017/746 Implementation/Impact, IVD/CDx/CLIA Approved Assays, High Dimensional Cytometry, Multiplexing Technologies, LBA Tissue Analysis, Vaccine Study Endpoints, Cell-Based Assays for Biomarkers, Cell Therapy and Vaccines ( PART 2 – Recommendations on Development & Validation of Biomarkers, IVD, CDx, Cell-Based, Flow Cytometry, Ligand-Binding and Enzyme Assays; Advanced Critical Reagents Strategies). Kholmanskikh O, Wang Y-M, Hersey S, Wadhwa M, Block K, Abbas Bandukwala, Szapacs M, Weiner R, Awwad K, Dessy F, et al. 2024 May 22. Bioanalysis.:1–42. doi:10.1080/17576180.2024.2340961.
Digital detection of proteins. Duffy DC. 2023. Lab on a Chip. 23(5):818–847. doi:10.1039/d2lc00783e.
A comprehensive review of Dynamic Chemical Labelling on Luminex xMAP technology: a journey towards Drug-Induced Liver Injury testing. Marín-Romero A, Pernagallo S. 2023. Analytical Methods. doi:10.1039/D3AY01481A.
A highly sensitive and quantitative assay for dystrophin protein using Single Molecule Count Technology. Ishii MN, Quinton M, Kamiguchi H. 2023. Neuromuscular Disorders. 33(10):737–743. doi:10.1016/j.nmd.2023.08.009.
Digital and Absolute Assays for Low Abundance Molecular Biomarkers. Kuo C-W, Smith A. 2023. Accounts of Chemical Research. 56(9):1031–1042. doi:10.1021/acs.accounts.3c00030.
Single-molecule immunoassay technology: Recent advances. Wu Y, Fu Y, Guo J, Guo J. 2023. Talanta. 265:124903. doi:10.1016/j.talanta.2023.124903.
Long COVID as a Tauopathy: Of “Brain Fog” and “Fusogen Storms.” Adonis Sfera, Rahman L, Manuel C, Zisis Kozlakidis. 2023. International Journal of Molecular Sciences. 24(16):12648–12648. doi:10.3390/ijms241612648.
Tralokinumab treatment improves the skin microbiota by increasing the microbial diversity in adults with moderate-to-severe atopic dermatitis: Analysis of microbial diversity in ECZTRA 1, a randomized controlled trial. Beck LA, Bieber T, Weidinger S, Tauber M, Saeki H, Irvine AD, Eichenfield LF, Werfel T, Arlert P, Jiang L, et al. 2022. Journal of the American Academy of Dermatology. doi:10.1016/j.jaad.2022.11.047.
Open a new window in the world of circulating microRNAs by merging ChemiRNA Tech with a Luminex platform. Marín-Romero A, Tabraue-Chávez M, W. Dear J, José Díaz-Mochón J, Pernagallo S. 2022. Sensors & Diagnostics. 1(6):1243–1251. doi:10.1039/D2SD00111J.
Ultra-sensitive AAV capsid detection by immunocapture-based qPCR following factor VIII gene transfer. Sandza K, Clark A, Koziol E, Akeefe H, Yang F, Holcomb J, Patton K, Hammon K, Mitchell N, Wong WY, et al. 2021. Gene Therapy. 29(1-2):94–105. doi:10.1038/s41434-021-00287-1.
The role of ligand-binding assay and LC–MS in the bioanalysis of complex protein and oligonucleotide therapeutics. Kotapati S, Deshpande M, Jashnani A, Thakkar D, Xu H, Dollinger G. 2021. Bioanalysis. 13(11):931–954. doi:10.4155/bio-2021-0009.
Quantitation of low abundant soluble biomarkers using high sensitivity Single Molecule Counting technology. Hwang J, Banerjee M, Venable AS, Walden Z, Jolly J, Zimmerman C, Adkisson E, Xiao Q. 2019. Methods. 158:69–76. doi:10.1016/j.ymeth.2018.10.018.
Standardized Immunomonitoring: Separating the Signals from the Noise. Duffy D. 2018. Trends in Biotechnology. 36(11):1107–1115. doi:10.1016/j.tibtech.2018.06.002.
Assay Formats: Recommendation for Best Practices and Harmonization from the Global Bioanalysis Consortium Harmonization Team. Dudal S, Baltrukonis D, Crisino R, Goyal MJ, Joyce A, Österlund K, Smeraglia J, Taniguchi Y, Yang J. 2013. The AAPS Journal. 16(2):194–205. doi:10.1208/s12248-013-9552-9.
Ultrasensitive Flow-based Immunoassays Using Single-Molecule Counting. Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P. 2007. Clinical Chemistry. 53(11):1990–1995. doi:10.1373/clinchem.2007.091181.

Metabolism/Endocrinology

Highly sensitive single-molecule counting technology for cytokeratin 18 detection in non-alcoholic fatty liver disease using novel antibodies. Liu P, Ben H, Guo J, Pang L, Liu N, Wang Y, Shi Y, Yang P, Gao Y, Guan Y, et al. 2024 Feb 1. Microchemical Journal.:110155–110155. doi:10.1016/j.microc.2024.110155.
Sarm1 knockout prevents type 1 diabetic bone disease in females independent of neuropathy. JCI Insight. Brazill JM, Shen IR, Craft CS, Magee KL, Park JS, Lorenz M, Strickland A, Wee NK, Zhang X, Beeve AT, et al. 2024. 9(4). doi:10.1172/jci.insight.175159.
Adipocyte NMNAT1 expression is essential for nuclear NAD+ biosynthesis but dispensable for regulating thermogenesis and whole-body energy metabolism. Yamaguchi S, Kojima D, Iqbal T, Kosugi S, Franczyk MP, Qi N, Sasaki Y, Yaku K, Kaneko K, Kinouchi K, et al. 2023. Biochemical and Biophysical Research Communications. 674:162–169. doi:10.1016/j.bbrc.2023.07.007.
Removal of Epididymal Visceral Adipose Tissue Prevents Obesity-Induced Multi-organ Insulin Resistance in Male Mice. Franczyk MP, He M, Yoshino J. 2021. Journal of the Endocrine Society. 5(5). doi:10.1210/jendso/bvab024.

Cancer

GDF-15 Neutralization Alleviates Platinum-Based Chemotherapy-Induced Emesis, Anorexia, and Weight Loss in Mice and Nonhuman Primates. Breen DM, Kim H, Bennett D, Calle RA, Collins S, Esquejo RM, He T, Joaquim S, Joyce A, Lambert M, et al. 2020. Cell Metabolism. 32(6):938-950.e6. doi:10.1016/j.cmet.2020.10.023.

Kidney Injury

Variation in renal responses to exercise in the heat with progressive acclimatisation. Omassoli J, Hill NE, Woods DR, Delves SK, Fallowfield JL, Brett SJ, Wilson D, Corbett RW, Allsopp AJ, Stacey MJ. 2019.Journal of Science and Medicine in Sport. 22(9):1004–1009. doi:10.1016/j.jsams.2019.04.010.

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What Is SMC® Technology?: An Overview of Ultrasensitive Immunoassay Technology

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SMC® Technology Workflow

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