UHPLC Analysis of Fat Soluble Vitamins on Titan C18, Acetonitrile Mobile Phase

column

TITAN C18, 50 mm x 2.1mm I.D. x 1.9 μm particles (577122-U)

mobile phase

100% acetonitrile

flow rate

0.4 mL/min

pressure

1755 psi (121 bar)

column temp.

30 °C

detector

UV, 280 nm

injection

0.5 μL

sample

All compounds at 0.5 mg/mL except retinyl acetate at 0.05 mg/mL in methanol