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UHPLC Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl

UHPLC Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl application for UHPLC

Materiales

columna analítica

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Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

componente de la fase móvil

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Ácido fosfórico

≥85 wt. % in H2O, ≥99.999% trace metals basis

patrón

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α-Boswellic acid

analytical standard

β-Boswellic acid

analytical standard

11-Keto-β-boswellic acid

analytical standard

3-O-Acetyl-11-keto-β-boswellic acid

analytical standard

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (53345-U)

mobile phase

[A] 0.1% phosphoric acid; [B] acetonitrile; (10:90, A:B)

flow rate

0.4 mL/min

pressure

850 psi (59 bar)

column temp.

35 °C

detector

UV, 210 nm

injection

2 μL

sample

6 Boswellic Acids 50μg/mL in 70:30, water:methanol

Descripción

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany