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LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column

LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column application for LC-MS

Materiales

Para utilizar con

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Precios

29654-U

Certified Vial Kit, Low Adsorption (LA), 2 mL, pk of 100

volume 2 mL, amber glass vial (with marking spot), natural PTFE/silicone septa (with slit), thread for 9 mm

columna analítica

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Precios

56401AST

Columna para HPLC apHera™ NH2

5 μm particle size, L × I.D. 15 cm × 4.6 mm

patrón

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Precios

45520

Glufosinate-ammonium

PESTANAL®, analytical standard

89432

N-(Phosphonomethyl)glycine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

MET12133A

(Aminomethyl)phosphonic acid

analytical standard

CONDITIONS

column

apHera NH2, 15 cm x 2 mm I.D., 5 μm particles (56400AST)

mobile phase

[A] water; [B] 20mM ammonium carbonate pH 9.0

gradient

100%A for 0.5 min; to 100% B in 0.1 min; held at 100% B for 6.4 min; to 100% A in 0.1 min; held at 100% A for 3 min

flow rate

0.3 mL/min

pressure

1570 psi (108 bar)

column temp.

30 °C

detector

ESI-MS(-); MS/MS 168/63; 110/63; 180/63

injection

15 μL

sample

50 ng/mL each compound in 50:50 methanol:water with 0.05% formic acid

Descripción

Nota de análisis

An LC/MS method for analysis of glyphosate, AMPA and glufosinate using MS-compatible mobile phase on an ion-exchange pH-stable HPLC column with mass spectrometry detection is shown here.

Información legal

apHera is a trademark of Sigma-Aldrich Co. LLC

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