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LC/MS (TOF) Analysis of Peonidin Glycosides from High Bush Blueberries on Ascentis® Express C18

LC/MS (TOF) Analysis of Peonidin Glycosides from High Bush Blueberries on Ascentis® Express C18 application for LC-MS

Materiales

columna analítica

Referencia del producto
Descripción
Precios

Columna para HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 0.1% (v/v) formic acid in water; [B] 0.1% (v/v) formic acid in 75:25 (v/v) acetonitrile:water

flow rate

0.2 mL/min

gradient

2% B for 2 min, to 100% B in 38 min

column temp.

35 °C

detector

ESI(+) TOF, extracted ions m/z 463.1244, 433.1140, 505.137

injection

1 μL

Descripción

Nota de análisis

The purpose of this study was to evaluate the change in anthocyanins levels in wild blueberries as a function of berry development. The delphinidin, putinidin, peonidin, and malvinidin base-modified components exhibited a dramatic increase in abundance as a function of berry development. The cyanidin base-modified components  exhibited a much lower increase in abundance as a function of berry development, it is uncertain the significance of the rate increase. Chlorogenic acid exhibited a continuous decrease in abundance as a function of berry development. Overall, the combination of UV/Vis detection along with accurate mass TOF enabled a effective method of identification of anthocyanins from blueberry extracts. The Ascentis Express column provided rapid, sensitive detection and rugged operation.

Otras notas

Berries (1.0 g) were added to 1.0 mL of 1% (v/v) formic acid in methanol. Samples were crushed in the solvent mixture and extracted (refrigerated) for 2 hours. A portion of the extracted sample was removed, centrifuged and the supernatant was collected for HPLC analysis.

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany