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LC/MS (TOF) Analysis of Dermorphin on Ascentis® Express F5

LC/MS (TOF) Analysis of Dermorphin on Ascentis® Express F5 application for HPLC

Materiales

columna analítica

Referencia del producto
Descripción
Precios

53569-U

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

2 mM ammonium formate (90:10 acetonitrile:water) adjusted to pH 4.5 with formic acid

flow rate

0.4 mL/min

column temp.

35 °C

detector

ESI(+), TIC 100 – 1000 m/z; APCI (+), XIC m/z = 150.1, 203.7, 378.2

injection

1 μL

sample

10 μg/mL in 75:25 acetonitrile:water

Descripción

Nota de análisis

Dermorphin is a heptapeptide derived from frogs. It is a painkiller and used illicitly in racehorses so they continue to run through the pain. A rapid analysis of dermorphin standard is shown here on an Ascentis Express F5 column. The HILIC mode separation provided the necessary retention and MS-compatibility, while the pentafluorophenyl (F5) phase chemistry elimated the chelating interactions that are problematic on bare silica.

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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