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HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18 application for HPLC

Materiales

Estándar

Referencia del producto
Descripción
Precios

Colecalciferol (D3)

analytical standard

Ergocalciferol (D2)

analytical standard

columna analítica

Referencia del producto
Descripción
Precios

Columna para HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

CONDITIONS

column

Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)

mobile phase

acetonitrile

flow rate

0.15 mL/min

column temp.

35 °C

detector

UV, 290 nm

injection

10 μL

sample

10 mg/L each (vitamins D2 and D3) in methanol

Descripción

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany