HPLC Analysis of Tetracycline Antibiotics on Ascentis® Express C18
Materiales
columna analítica
Referencia del producto
Descripción
Precios
componente de la fase móvil
Referencia del producto
Descripción
Precios
patrón
Referencia del producto
Descripción
Precios
precolumna
Referencia del producto
Descripción
Precios
Ascentis® Express C18, 2.7 Micron Guard Cartridge
2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 eatambién adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Screw Top Vial, Silane-Treated
volume 2 mL, amber glass vial, O.D. × H × I.D. 12 mm × 32 mm × 4.6 mmCONDITIONS
column
Ascentis Express C18, 15 cm x 3.0 mm I.D., 2.7 μm particles (53816-U)
mobile phase
[A] 10 mM oxalic acid in water; [B] 5 mM oxalic acid in methanol:acetonitrile (1:1)
gradient
15% B for 1 min; to 20% B in 0.1 min, held at 20% for 4 min; to 80% B in 5 min; to 15% B in 0.1 min, held at 15% B for 3 min
flow rate
0.5 mL/min
pressure
3597 psi (248 bar)
column temp.
30 °C
detector
UV, 355 nm
injection
50 μL
sample
100 ng/mL of each compound in acetontrile:water:formic acid (5:94.9:0.1)
Descripción
Nota de análisis
Separation of tetracycline antibiotics from their epi-metabolites was achieved on an Ascentis® Express C18 HPLC column. Oxalic acid was added to the mobile phase to prevent the loss of these metal-binding compounds in the HPLC system.
Información legal
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany