HPLC Analysis of Paraquat and Diquat on Ascentis^® Express HILIC (UV Detection)

column

Ascentis Express HILlC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

[A] 200 mM ammonium TFA; [B] acetonitrile; (20:80, A:B)

flow rate

0.4 mL/min

column temp.

60 °C

detector

UV, 257 nm (paraquat) and 308 nm (diquat)

injection

1 μL

sample

50 mg/L in mobile phase