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HPLC Analysis of Organic Acids Commonly Found in Wine and Beer on TSKgel® OApak-P and OApak-A

HPLC Analysis of Organic Acids Commonly Found in Wine and Beer on TSKgel® OApak-P and OApak-A application for HPLC

Materiales

columna analítica

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TSKgel® OApak-A HPLC Column

phase carboxylic acid, L × I.D. 30 cm × 7.8 mm, 7 μm particle size

componente de la fase móvil

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Sulfuric acid concentrate

0.1 M H2SO4 in water (0.2N), eluent concentrate for IC

patrón

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L-Pyroglutamic acid

≥99.0% (T)

Ácido cítrico

United States Pharmacopeia (USP) Reference Standard

Ácido L-(+)-Láctico

United States Pharmacopeia (USP) Reference Standard

Malic acid

United States Pharmacopeia (USP) Reference Standard

Ácido succínico

United States Pharmacopeia (USP) Reference Standard

Tartaric acid

United States Pharmacopeia (USP) Reference Standard

Ácido pirúvico

United States Pharmacopeia (USP) Reference Standard

precolumna

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TSKgel® OApak-P Guard Column

phase sulphonic acid, L × I.D. 4 cm × 6 mm, 10 μm particle size

CONDITIONS

column

TSKgel OApak-A, 30 cm x 7.8 mm I.D., 5 μm particles (816653)

column

TSKgel OApak-P Guard Column, 4 cm x 6 mm I.D., 10 μm particles (816654)

mobile phase

0.75 mM sulfuric acid

flow rate

0.8 mL/min

column temp.

60 °C

detector

conductivity

injection

20 μL

sample

organic acid mixture, see figure for concentrations

Descripción

Otras notas

Data provided by Tosoh Bioscience LLC.

Información legal

TSKgel is a registered trademark of Tosoh Corporation