HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5
Materiales
columna analítica
Referencia del producto
Descripción
Precios
patrón
Referencia del producto
Descripción
Precios
Morphine-3-β-D-glucuronide solution
100 μg/mL in methanol: water ( 50:50(v/v)), ampule of 1 mL, certified reference material, Cerilliant®Morphine solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Morphine-6-β-D-glucuronide solution
100 μg/mL in methanol: water (1:1), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Discovery HS F5, 5 cm x 4.6 mm I.D., 5 μm particles (567513-U)
mobile phase
[A] 10 mM ammonium acetate, pH 6.8 (unadjusted); [B] acetonitrile
gradient
0% B for 1 min, to 30% B in 4 min, held 3 min
flow rate
1 mL/min
column temp.
40 °C
detector
UV, 280 nm
injection
10 μL
sample
50 μg/mL in final mobile phase
Descripción
Información legal
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany