HPLC Analysis of Heroin, Cocaine, Amphetamine and MDMA on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

[A] 10 mM ammonium acetate in water, pH 3.5; [B] 10 mM ammonium acetate in acetonitrile; (2.98, A:B)

flow rate

0.4 mL/min

column temp.

50 °C

detector

ESI(+), m/z 100-400

injection

1 μL