HPLC Analysis of Beverage Additives in Diet Cola on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] 20 mM ammonium acetate, pH 4.7 with acetic acid: [B] acetonitrile; (90:10, A:B)

flow rate

0.6 mL/min

column temp.

40 °C

detector

UV, 214 nm

injection

1 μL

sample

100 - 500 μg/mL in 95:5, buffer: acetonitrile