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Hydroxylation of a conserved tRNA modification establishes non-universal genetic code in echinoderm mitochondria.

Nature structural & molecular biology (2017-08-08)
Asuteka Nagao, Mitsuhiro Ohara, Kenjyo Miyauchi, Shin-Ichi Yokobori, Akihiko Yamagishi, Kimitsuna Watanabe, Tsutomu Suzuki
RESUMEN

The genetic code is not frozen but still evolving, which can result in the acquisition of 'dialectal' codons that deviate from the universal genetic code. RNA modifications in the anticodon region of tRNAs play a critical role in establishing such non-universal genetic codes. In echinoderm mitochondria, the AAA codon specifies asparagine instead of lysine. By analyzing mitochondrial (mt-) tRNA

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C75, ≥98% (HPLC), powder