Saltar al contenido
Merck
  • Effect of oral N-acetyl cysteine supplementation in type 2 diabetic patients on intracellular glutathione content and innate immune responses to Burkholderia pseudomallei.

Effect of oral N-acetyl cysteine supplementation in type 2 diabetic patients on intracellular glutathione content and innate immune responses to Burkholderia pseudomallei.

Microbes and infection (2014-08-05)
Akshamal M Gamage, Kok Onn Lee, Yunn-Hwen Gan
RESUMEN

Type 2 diabetic patients have increased susceptibility to melioidosis, an infectious disease caused by Burkholderia pseudomallei. We had previously shown that peripheral blood mononuclear cells (PBMCs) from diabetic patients with poor glycemic control had a defective IL-12 and IFNγ response to B. pseudomallei infection, resulting in poor intracellular bacterial control. The impaired IL-12 response was due to glutathione (GSH) deficiency characterized by a low reduced to oxidized glutathione ratio (GSH ratio) and could be restored by the addition of reduced GSH to the infected cells. Our goal is to determine whether N-acetyl cysteine (NAC, a GSH pro-drug) supplementation in diabetic patients could improve their immune control of B. pseudomallei. Type 2 diabetic patients with poor glycemic control were given oral supplementation of NAC for six weeks at 1200 mg daily. Their PBMCs and subsets of immune cells showed a significant increase in free GSH concentration. However, the GSH ratio, IL-12 and IFNγ production, and intracellular bacterial killing upon ex-vivo infection did not improve. Thus, oral NAC supplementation in diabetic patients is sufficient to increase intracellular GSH content in blood cells. However, modulating the free GSH content is not sufficient to improve infection outcome as it is the GSH ratio that regulates the IL-12 response in monocytes.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
L-Glutatión reducido, suitable for cell culture, BioReagent, ≥98.0%, powder
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
2-Vinylpyridine, 97%
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
Diethoxymethane, 99.7%, contains 50-150 ppm BHT as stabilizer
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
L-Glutatión reducido, ≥98.0%
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
Formaldehyde diethyl acetal, absolute, over molecular sieve (H2O ≤0.01%), ≥99.0% (GC)
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Supelco
L-Glutatión reducido, Pharmaceutical Secondary Standard; Certified Reference Material
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
L-Glutatión reducido, BioXtra, ≥98.0%
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
L-Glutatión reducido, European Pharmacopoeia (EP) Reference Standard
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad