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A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

Organic & biomolecular chemistry (2014-12-17)

S Bag, J-C Tseng, J Rochford

RESUMEN

Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.

MATERIALES

Referencia del producto

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Descripción del producto

Sigma-Aldrich

Tetrahidrofuran, inhibitor-free, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Tetrahidrofuran, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%

Sigma-Aldrich

Forbol 12-miristato 13-acetato, ≥99% (TLC), film or powder

Sigma-Aldrich

Borohidruro de sodio, powder, ≥98.0%

Sigma-Aldrich

Borohidruro de sodio, ReagentPlus^®, 99%

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Triethylamine, ≥99.5%

Sigma-Aldrich

p-Toluenesulfonic acid monohydrate, ≥99% (calc. to H₂O free subst.)

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, ≥99%

Sigma-Aldrich

Luminol, 97%

Sigma-Aldrich

Borohidruro de sodio, granular, 99.99% trace metals basis

Sigma-Aldrich

2,3-Dichloro-5,6-dicyano-p-benzoquinone, 98%

Sigma-Aldrich

p-Toluenesulfonic acid monohydrate, ACS reagent, ≥98.5%

Sigma-Aldrich

Triethylamine, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

Borohidruro de sodio, purum p.a., ≥96% (gas-volumetric)

Sigma-Aldrich

Boron, ≥95% (boron), amorphous powder

Sigma-Aldrich

Tetrahidrofuran, ACS reagent, ≥99.0%, contains 250 ppm BHT as inhibitor

Sigma-Aldrich

Forbol 12-miristato 13-acetato, synthetic, ≥98.0% (TLC)

Sigma-Aldrich

Luminol, ≥97% (HPLC)

Sigma-Aldrich

Sodium borohydride solution, ~12 wt. % in 14 M NaOH

Sigma-Aldrich

p-Toluenesulfonic acid monohydrate, ReagentPlus^®, ≥98%

Sigma-Aldrich

Borohidruro de sodio, granular, 10-40 mesh, 98%

Supelco

Tetrahidrofuran, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, ≥99.5%

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Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

Sigma-Aldrich

Boron, crystalline, 1 cm, 99.7% trace metals basis

Supelco

Tetrahidrofuran, analytical standard

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Borohidruro de sodio, caplets (18 × 10 × 8 mm), 98%

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Sodium borohydride solution, 2.0 M in triethylene glycol dimethyl ether