Saltar al contenido
Merck

Biochemical characterization of IMP-30, a metallo-β-lactamase with enhanced activity toward ceftazidime.

Antimicrobial agents and chemotherapy (2013-07-10)
Kevin M Pegg, Eleanor M Liu, Alecander E Lacuran, Peter Oelschlaeger
RESUMEN

IMP-type enzymes constitute a clinically important family of metallo-β-lactamases that has grown dramatically in the past decade to its current 45 known members. Here, we report the biochemical characterization of IMP-30 in comparison to IMP-1, from which it deviates by a single E59K mutation. Kinetics, MIC assays, docking, and molecular dynamics simulations support a scenario in which Lys59 interacts with the ceftazidime R1 group, resulting in increased water access and enhanced turnover and MIC of ceftazidime.

MATERIALES
Referencia del producto
Marca
Descripción del producto

USP
Ceftazidime pentahydrate, United States Pharmacopeia (USP) Reference Standard
Ceftazidime, European Pharmacopoeia (EP) Reference Standard
Ceftazidime for peak identification, European Pharmacopoeia (EP) Reference Standard