Saltar al contenido
Merck
Todas las fotos(1)

Documentos

SRP6437

Sigma-Aldrich

CD55/DAF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Sinónimos:

CR, CROM, TC

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

origen biológico

human

recombinante

expressed in HEK 293 cells

etiqueta

6-His tagged (C-terminus)

Análisis

≥95% (SDS-PAGE)

formulario

lyophilized

mol peso

calculated mol wt 35.8 kDa
observed mol wt 50-65 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 35 is the predicted N-terminal.)

envase

pkg of 10 μg
pkg of 50 μg

Nº de acceso UniProt

P08174

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CD55(1604)

Descripción general

CD55 (complement decay-accelerating factor), also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells.

Acciones bioquímicas o fisiológicas

CD55 (complement decay-accelerating factor) prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first short consensus repeats (SCR). It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C′ attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

Forma física

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Molecular basis of reduced or absent expression of decay-accelerating factor in Cromer blood group phenotypes.
Lublin D M, et al.
Blood, 84(4), 1276-1282 (1994)
Structure of complement fragment C3b?factor H and implications for host protection by complement regulators.
Wu J, et al.
Nature Immunology, 10(7), 728-728 (2009)
Membrane complement regulatory proteins.
Kim D D and Song W C
Clinical Immunology (Orlando, Fla.), 118(2-3), 127-136 (2006)
Hantavirus causing hemorrhagic fever with renal syndrome enters from the apical surface and requires decay-accelerating factor (DAF/CD55).
Krautkramer E and Zeier M
Journal of Virology, 82(9), 4257-4264 (2008)
An understanding of intervertebral disc development, maturation and cell phenotype provides clues to direct cell-based tissue regeneration therapies for disc degeneration.
Rodrigues-Pinto R, et al.
European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 23(9), 1803-1814 (2014)
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico