SRP4515

Artemin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

• Sinónimos: ART, ARTN, EVN, NBN
• Número MDL: MFCD03099559
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Análisis: ≥98% (SDS-PAGE)
• formulario: lyophilized
• potencia: 2-5 ng/mL ED₅₀ (it′s ability to stimulate the proliferation of human SH-SY5Y cells)
• mol peso: ~24 kDa
• envase: pkg of 20 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• impurezas: endotoxin, tested
• Nº de acceso NCBI: NM_057090
• Nº de acceso UniProt: Q5T4W7
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... ARTN(9048)

Descripción

Descripción general

Human Artemin, a neurotrophic factor, belongs to the GDNF family. Artemin is a survival factor for sensory and sympathetic neurons in culture. Recombinant human Artemin is a 24.2 kDa, disulfide-linked homodimer formed by two identical 113 amino acid subunits. The gene encoding this protein is localized on human chromosome 1p34.1.

Acciones bioquímicas o fisiológicas

Artemin (ART/ARTN) is a survival factor for sensory and sympathetic neurons in culture. It has a role in promoting survival of neurons. ART is also involved in the differentiation and development of the central and peripheral nervous systems. The protein promotes axonal projection and migration in sympathetic neurons. Artemin restores neural damage and diminishes neuropathic pain. It has been associated with various carcinomas.

Forma física

Lyophilized from 10 mM Sodium Citrate pH 4.5 plus 150 mM NaCl.

Nota de preparación

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitución

Reconstitute in water to a concentration of 0.1-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable