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Merck

MAK417

Sigma-Aldrich

Glycogen Phosphorylase Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Código UNSPSC:
12161503

método de detección

colorimetric

enfermedades relevantes

cancer

temp. de almacenamiento

−20°C

Descripción general

Glycogen phosphorylase catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms. Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is a clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes.

Características y beneficios

Glycogen Phosphorylase Assay Kit:
  • Detects as low as 10 mU in a variety of sample types.

Idoneidad

The kit is suitable for the measurement of glycogen phosphorylase activity in cell culture and animal tissues (heart, liver, kidney, muscle, etc.) as well as the analysis of glycogen phosphorylase kinetics, inhibition or activation.

Principio

The Glycogen Phosphorylase Colorimetric Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at A450 nm. The A450 nm signal is directly proportional to the glycogen phosphorylase activity. The kit is a rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity.

Pictogramas

Health hazardCorrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3


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